Autor(es): Fernandes, Andreia R. ; Martins, João P. ; Gomes, Edgar R. ; S. Mendes, César ; Teodoro, Rita
Data: 2023
Identificador Persistente: http://hdl.handle.net/10362/154150
Origem: Repositório Institucional da UNL
Assunto(s): Chemistry(all); Biochemistry, Genetics and Molecular Biology(all); Physics and Astronomy(all)
Funding Information: We would like to thank Telmo Pereira from the Microscopy Facility for technical support, the Fly Facility at Nova Medical Research; CONGENTO: consortium for genetically tractable organisms. We thank the Developmental Studies Hybridoma Bank, Bloomington Drosophila Stock Center and VDRC for antibodies and fly stocks. This work was supported by PTDC-01778/2022- NeuroDev3D to R.O.T. GEMiNI and PTDC/BIA-COM/0151/2020 to C.S.M and European Research Council H2020-GA 810207-ARPCOMPLEXITY to E.R.G. A.R.F. is supported with a PhD scholarship from Fundação para a Ciência e Tecnologia, Portugal, reference SFRH/BD/144488/2019, and J.P.M. with a reference SFRH/BD/130920/2017. This work also supported by iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020), and LS4FUTURE (LA/P/0087/2020). Funding Information: We would like to thank Telmo Pereira from the Microscopy Facility for technical support, the Fly Facility at Nova Medical Research; CONGENTO: consortium for genetically tractable organisms. We thank the Developmental Studies Hybridoma Bank, Bloomington Drosophila Stock Center and VDRC for antibodies and fly stocks. This work was supported by PTDC-01778/2022- NeuroDev3D to R.O.T. GEMiNI and PTDC/BIA-COM/0151/2020 to C.S.M and European Research Council H2020-GA 810207-ARPCOMPLEXITY to E.R.G.. A.R.F. is supported with a PhD scholarship from Fundação para a Ciência e Tecnologia, Portugal, reference SFRH/BD/144488/2019, and J.P.M. with a reference SFRH/BD/130920/2017. This work also supported by iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020), and LS4FUTURE (LA/P/0087/2020). Publisher Copyright: © 2023, The Author(s).
Wired neurons form new presynaptic boutons in response to increased synaptic activity, however the mechanism(s) by which this occurs remains uncertain. Drosophila motor neurons (MNs) have clearly discernible boutons that display robust structural plasticity, being therefore an ideal system in which to study activity-dependent bouton genesis. Here, we show that in response to depolarization and in resting conditions, MNs form new boutons by membrane blebbing, a pressure-driven mechanism that occurs in 3-D cell migration, but to our knowledge not previously described to occur in neurons. Accordingly, F-actin is decreased in boutons during outgrowth, and non-muscle myosin-II is dynamically recruited to newly formed boutons. Furthermore, muscle contraction plays a mechanical role, which we hypothesize promotes bouton addition by increasing MN confinement. Overall, we identified a mechanism by which established circuits form new boutons allowing their structural expansion and plasticity, using trans-synaptic physical forces as the main driving force.
