Author(s):
Rosa, Ricardo ; Marques, Cláudia ; Rodrigues, Olivia Roos ; Santos-Gomes, Gabriela M.
Date: 2006
Persistent ID: http://hdl.handle.net/10362/157433
Origin: Repositório Institucional da UNL
Subject(s): CD4 T cells; CD8 T cells; Cytokine; L. infantum released proteins; Rodent model; RA0421 Public health. Hygiene. Preventive Medicine; QR Microbiology; QR180 Immunology; Parasitology; veterinary (miscalleneous); Insect Science; Infectious Diseases; Immunology and Microbiology (miscellaneous); SDG 1 - No Poverty; SDG 3 - Good Health and Well-being
Description
Funding Information: The authors thank J.M. Cristóvão and J. Ramada for technical assistance. Funding for this work was provided by the project research grant POCTI/CVT/37759/2001 by the Fundação para a Ciência e Tecnologia (FCT), Portugal partially supported by the European Union Fund (FEDER), and grant SFRH/BD/997/2000 from FCT.
Specific immune responses by CD4+ and CD8+ T cells, from two infected mice strains (BALB/c and C57BL/6), induced by High, Inter and Low protein fractions released by Leishmania infantum, were assessed through the evaluation of IL-12, IFN-γ and IL-10 mRNA by real-time PCR and respective protein production by ELISA. During infection establishment, High and Inter fractions directed both mice strains T cells subsets to increase the production of IFN-γ, associated to IL-12 release. Later on, parasite replication augmented in BALB/c and stabilised in C57BL/6 mice. Inter fraction induced CD4+ T cells to maintain IFN-γ production, with the simultaneous release of IL-12 by both cell subsets in BALB/c mice and by CD8+ T cells in C57BL/6 mice. These observations suggested a prophylactic potential for Inter fraction which was able to induce Th1 response with IL-12 involvement, required for the maintenance of memory cells, in mice strains with different parasitic evolution.
