Autor(es): Encarnação, Sofia ; Lima, Katelene ; Malú, Quintino ; Caldeira, Gonçalo I. ; Duarte, Maria Paula ; Rocha, João ; Lima, Beatriz Silva ; Silva, Olga
Data: 2024
Identificador Persistente: http://hdl.handle.net/10362/172401
Origem: Repositório Institucional da UNL
Assunto(s): cashew; ethnopharmacology; free radical scavenger; inflammation; phytochemistry; pre-clinical safety; Ecology, Evolution, Behavior and Systematics; Ecology; Plant Science; SDG 3 - Good Health and Well-being
Funding Information: This research was funded by Foundation for Science and Technology (FCT, Portugal) through national funds FCT/MCTES to iMed.ULisboa (UIDP/04138/2020). Publisher Copyright: © 2024 by the authors.
Anacardium occidentale L. stem bark Traditional Herbal Preparations (AoBTHPs) are widely used in traditional medicine to treat inflammatory conditions, such as diabetes. The present study aims to evaluate the anti-inflammatory, antioxidant, and genotoxic potential of red and white Portuguese AoBTHPs. Using a carrageenan-induced rat paw edema model, a significant anti-edema effect was observed for all tested doses of white AoBTHP (40.2, 71.5, and 127.0 mg/kg) and the two highest doses of red AoB THP (71.5 and 127.0 mg/kg). The anti-edema effect of red AoBTHP’s highest dose was much more effective than indomethacin 10 mg/kg, Trolox 30 mg/kg, and Tempol 30 mg/kg. In DPPH, FRAP, and TAC using the phosphomolybdenum method, both types of AoBTHPs showed similar antioxidant activity and no genotoxicity up to 5000 µg/plate in the Ames test. The LC-UV/DAD-ESI/MS fingerprint allowed the identification of gallic and protocatechuic acids as the two main marker compounds and the presence of catechin, epicatechin, epigallocatechin gallate, and ellagic acid in both AoBTHPs. The obtained results support the validation of red and white AoB and their THPs as anti-inflammatory agents and contribute to the possible development of promising new therapeutic options to treat inflammatory conditions.
