Autor(es):
Pars, Selin ; Cristo, Fernando ; Inácio, José M ; Rosas, Graça ; Carreira, Isabel Marques ; Melo, Joana Barbosa ; Mendes, Patrícia ; Martins, Duarte Saraiva ; de Almeida, Luís Pereira ; Maio, José ; Anjos, Rui ; A. Belo, José
Data: 2018
Identificador Persistente: http://hdl.handle.net/10362/37756
Origem: Repositório Institucional da UNL
Projeto/bolsa:
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F81431%2F2011/PT;
info:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04462%2F2013/PT;
Assunto(s): SDG 3 - Good Health and Well-being
Descrição
We would like to thank the patient and their guardians for their generous donation of the urine sample used in this study. We also would like to thank Ana Jardim for technical support in karyotype analysis. This work was supported by Fundacao para a Ciencia e a Tecnologia (PTDC/BIM-MED/3363/2014). iNOVA4Health - UID/Multi/04462/2013, a program financially supported by Fundacao para a Ciencia e Tecnologia/Ministerio da Educacao e Ciencia, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement is acknowledged.
A DAND5-control human iPSC line was generated from the urinary cells of a phenotypically normal donor. Exfoliated renal epithelial (RE) cells were collected and reprogrammed into iPSCs using Sendai virus reprogramming system. The pluripotency, in vitro differentiation potential, karyotype stability, and the transgene-free status of generated iPSC line were analyzed and confirmed. This cell line can be exploited as a control iPSC line to better understand the mechanisms involved in DAND5-associated cardiac disease.
