Author(s): Alberto, F. ; Whitmer, A. ; Coelho, N. C. ; Zippay, M. ; E, Varela-Álvarez ; Raimondi, P. T. ; Reed, D. C. ; Serrão, Ester
Date: 2009
Persistent ID: http://hdl.handle.net/10400.1/4040
Origin: Sapientia - Universidade do Algarve
Subject(s): Macrocystis pyrifera; Giant kelp; Microsatellites; Macroalga; Genetic diversity
We report the isolation and characterization of 16 microsatellite loci to study the population genetics of the giant kelp, Macrocystis pyrifera. Markers were obtained by screening a genomic library enriched for microsatellite motifs. Of the 37 primer pairs defined, 16 amplified clean polymorphic microsatellites and are described. These loci identified a number of alleles ranging from three to forty (mean = 16.5, and gene diversity ranging from 0.469 to 0.930 (mean = 0.774). The isolation and characterization of these highly polymorphic markers will greatly benefit much needed studies on the molecular ecology of this important macroalga.
