Autor(es): Carvalho,Rusbene Bruno Fonseca de ; Almeida,Antonia Amanda Cardoso de ; Freitas,Rivelilson Mendes de ; Lima,Luciano Silva ; David,Juceni Pereira ; David,Jorge Mauricio ; Feitosa,Chistiane Mendes
Data: 2013
Origem: Oasisbr
Assunto(s): Citrus limon (L.) Burm; psoraleno; acetylcholinesterase
This paper describes the chemical composition and acetylcholinesterase inhibition of an active chromatographic fraction of the EtOAc extract of Citrus limon leaves. The composition of the active fraction presented a mixture of two coumarins, 5,8-dimethoxy-psoralen and 5,7- dimethoxycoumarin, identified by ¹H and ¹³C NMR data analysis, including DEPT, COSY, HMBC and HSQC experiments. It was also demonstrated that this mixture presents qualitative and quantitative AChE inhibition. In vitro studies indicated a CE50 value of 340 µg/mL with 95% of confidence. In vivo studies (10 and 25 mg/kg) revealed inhibition of 30.09 and 30.06% of AChE activity in relation to neostigmine, respectively.
