Author(s): Freitas,Jose Octavio Gonçalves de ; Quieregatto,Paulo Rogério ; Hochman,Bernardo ; Lapin,Guilherme Abbud Franco ; Mella,Stella Maria Botequio ; Maximino,Jessica Ruivo ; Chadi,Gerson ; Ferreira,Lydia Masako
Date: 2015
Origin: Oasisbr
Subject(s): Calcitonin Gene-Related Peptide; Substance P; Neuropeptides; Dexamethasone; Neurogenic Inflammation; Skin; Rats
PURPOSE: To investigate the neuropeptides substance P (SP) and calcitonin gene-related peptide (CGRP) after subcutaneous injection of dexamethasone prior to skin incision in rats.METHODS:Twenty seven Wistar-EPM-1 rats were randomly divided into three groups. The sham group (SG) of rats was injected with 0.9 % saline. The second group (Dexa) was injected with 1.0 mg/kg dexamethasone, and the third group (Dexa+) was injected with 10.0 mg/kg dexamethasone. In all groups, the three subcutaneous injections were performed 30 minutes prior to the surgical skin incision and tissue collection. SP and CGRP (15 kDa pro-CGRP and 5 kDa CGRP) were quantified by Western Blotting.RESULTS: No statistically significant differences (p>0.05) were found in pro-CGRP, CGRP and SP values in all three groups.CONCLUSION:The anti-inflammatory effect of dexamethasone did not occur when the substance P and calcitonin gene-related peptide levels were altered during the neurogenic inflammation process of skin wound healing in rats.
