Author(s): Miot,Hélio Amante ; Brianezi,Gabrielli ; Tamega,Andréia de Almeida ; Miot,Luciane Donida Bartoli
Date: 2012
Origin: Oasisbr
Subject(s): Cluster analysis; Image cytometry; Melanin; Melanosis; Pigmentation disorders
Document details
Techniques of digital image analysis for histological quantification of melanin
Description
Morphometric analysis of tissue melanin may quantitatively contribute to research on pigmentation disorders. The authors present three methods for image analysis, which allow for identification of melanin-equivalent pixels in the epidermis using Fontana-Masson stain and, therefore, for the calculation of its percentage in the different epidermal layers. Moreover, they discuss the main elements related to the analysis and the need for rigorous standardization of the process.
Document Type
Journal article
Language English
Language English
