Author(s): Barreto Neto, Laerte Oliveira
Date: 2019
Origin: Oasisbr
Subject(s): Citocinas; Doen?as Periodontais; Plantas Medicinais; Anti-Infecciosos; Cytokines; Periodontal Diseases; Plants Medicinal; Anti-infective agents; CIENCIAS BIOLOGICAS::BIOLOGIA GERAL; CIENCIAS BIOLOGICAS
Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2019-11-13T22:09:49Z No. of bitstreams: 1 Tese final Laerte.pdf: 4730378 bytes, checksum: a2c34124e294d4bd3f8c259f82b37cc3 (MD5)
Made available in DSpace on 2019-11-13T22:09:49Z (GMT). No. of bitstreams: 1 Tese final Laerte.pdf: 4730378 bytes, checksum: a2c34124e294d4bd3f8c259f82b37cc3 (MD5) Previous issue date: 2019-09-03
The use of herbal medicines has become a valuable resource in its treatment of oral disorders such as fungal and bacterial infections. One of the most prevalent infectious and inflammatory diseases in the mouth is periodontitis, which if left untreated, can lead to tooth loss. This study evaluated the immunomodulatory and antimicrobial potential of Lippia alnifolia Mart & Schauer (LA) and Vatairea macrocarpa (Benth.) Ducke (VM) leaf extract. Immunological evaluation was performed by immunoassay after culturing peripheral blood cells of individuals with and without periodontitis with the plant extract and the periodontopathogen Porphyromonas gingivalis (PG) extract. Antimicrobial activity was tested with minimal inhibitory concentration and disk diffusion assay. LA extract inhibited the production of IL-13, IL-6 and IFN-?, both in relation to the basal concentration produced by cells and the concentration produced by cells co-cultured with PG extract. Co-cultivation of MV with PG promoted a reduction in IL-1?, IL-13, IL-6, IFN-?, IL-10 and IL-17 levels. The LA extract showed bacteriostatic activity for Bacillus subtilis, Escherichia coli, Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus and Salmonella choleraesuis, fungistatic for Candida albicans and fungicide for Candida glabrata. VM showed bactericidal activity for Micrococcus luteus species and bacteriostatic other bacteria, as well as fungistatic activity for Candida albicans and fungicide for Candida glabrata. LA and VM extracts are promising in regulating cytokine production in PG antigens and in controlling oral microorganisms.
O uso de fitoter?picos tem se tornado um recurso valioso no seu tratamento de afec??es bucais, como infec??es f?ngicas e bacterianas. Uma das doen?as infecciosas e inflamat?rias mais prevalentes na boca ? a periodontite, que se n?o tratada, pode levar ? perda dent?ria. Este estudo avaliou o potencial imunomodulador e antimicrobiano do extrato das folhas de Lippia alnifolia Mart. & Schauer (LA) e Vatairea macrocarpa (Benth.)Ducke (VM). A avalia??o imunol?gica foi realizada por imunoensaio, ap?s cultivo das c?lulas do sangue perif?rico de indiv?duos com e sem periodontite com o extrato da planta e o extrato do periodontopat?geno Porphyromonas gingivalis (PG). A atividade antimicrobiana foi testada com concentra??o inibit?ria m?nima e ensaio de difus?o em disco. O extrato de LA inibiu a produ??o de IL-13, IL-6 e IFN-?, tanto em rela??o com a concentra??o basal produzida pelas c?lulas, quanto com a concentra??o produzida pelas c?lulas co-cultivadas com o extrato de PG. O co-cultivo de VM com PG promoveu uma redu??o nos n?veis de IL-1?, IL-13, IL-6, IFN- ?, IL-10 e IL-17. O extrato de LA apresentou atividade bacteriost?tica para Bacillus subtilis, Escherichia coli, Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus e Salmonella choleraesuis, fungist?tica para Candida albicans e fungicida para Candida glabrata. VM apresentou atividade bactericida para a esp?cie Micrococcus luteus e bacteriost?tica demais bact?rias, bem como atividade fungist?tica para Candida albicans e fungicida para Candida glabrata. Os extratos de LA e VM s?o promissores na regula??o da produ??o de citocinas em a ant?genos de PG e no controle de microrganismos bucais.
