Combination of different methods for detection of Campylobacter spp. in young children with moderate to severe diarrhea

• Autor(es): Veras, Herlice do Nascimento ; Quetz, Josiane da Silva ; Lima, Ila Fernanda Nunes ; Rodrigues, Tamara Silva ; Havt, Alexandre ; Rey, Luís Carlos ; Mota, Rosa Maria Salani ; Soares, Alberto Melo ; Singhal, Mitra ; Weigl, Bernhard ; Guerrant, Richard ; Lima, Aldo Angelo Moreira
• Data: 2017
• Origem: Oasisbr
• Assunto(s): Diarréia Infantil; Campylobacter
• Tipo de Documento: Artigo científico
• Idioma: Inglês

Descrição

Campylobacter spp. were detected - using culture, ELISA, PCR, and qPCR - among children (0 – 36 months) with moderate to severe diarrhea in Northeastern Brazil. Our data showed that either the qPCR alone or PCR along with ELISA might be an alternative to culture to diagnose Campylobacter due to their enhanced sensitivity.