Autor(es): Carvalho, Francisco Samuel Rodrigues ; Feitosa, Victor Pinheiro Feitosa ; Silva, Paulo Goberl^anio de Barros ; Soares, Eduardo Costa Studart ; Ribeiro, Thyciana Rodrigues ; Fonteles, Cristiane Sá Roriz ; Costa, Fábio Wildson Gurgel
Data: 2018
Origem: Oasisbr
Assunto(s): Microscopia Eletrônica de Varredura; Microscopy, Electron, Scanning
Purpose: To evaluate different therapeutic Carnoy's solution formulations on hard human tissues. Materials and methods: An in vitro study was performed with human teeth (n ¼ 36) and bone fragments (n ¼ 18), randomly divided into two experimental groups (Group I ¼ Carnoy solution with chloroform; Group II ¼ Carnoy solution without chloroform) and a control group (saline solution). The groups were subdivided into pre-conditioning, post-conditioning, and post-conditioning with saline washing. Raman microspectroscopy, Knoop microhardness test, and scanning electron microscopy with energy dispersive X-ray spectroscopy were used. Results: Therewas demineralization of dental structures regardingmineral/matrix and carbonate/phosphate ratios (GI versus GII, p < 0.05). The presence of chloroform resulted in a statistically significant reduction of the teeth surface microhardness (p ¼ 0.036), but not exceeding 0.01 mm. Both GI and GII showed significant structural changes by using scanning electron microscopy with energy dispersive X-ray spectroscopy. Conclusion: Carnoy's solution altered the organic and inorganic matrix of the human calcified specimens analyzed in vitro, and its effect was more pronounced when chloroform was present.
