Author(s): Alves Filho, Elenilson G. ; Silva, Lorena Mara A. ; Oiram Filho, Francisco ; Rodrigues, Sueli ; Fernandes, Fabiano A. N. ; Gallão, Maria Izabel ; Mattison, Christopher P. ; Brito, Edy S. de
Date: 2022
Origin: Oasisbr
Subject(s): Allergens; Anacardic acids; Anacardium occidentale; Chemometrics; NMR
The study investigated the influence of atmospheric plasma processing on cashew nut composition as well as on its allergenicity. The cashew nuts were processed by low-pressure plasma, using glow discharge plasma (80 W and 50 kHz power supply). Anacardic acids and allergens were quantified by HPLC and immunoassay, respec- tively. Additionally, the overall composition was evaluated by 1H qNMR. Increases in amounts of anacardic acids (15:1, 15:2, and 15:3) and fatty acids (oleic, linoleic, palmitic and stearic) were detected after all process conditions, with 70.92% of total variance captured using 2 LVs. The total amount of anacardic acids increased from 0.7 to 1.2 μg·mg−1 of nut. The major change was observed for anacardic acid (C15:3) with an increase from 0.2 to 0.55 μg/mg of nut for the samples treated with a flow of 10 mL·min−1 and 30 min of processing. On the other hand, the amount of sucrose decreased, from 33 to 18 mg·g−1 of nut, after all processing conditions. Plasma processing of cashew nuts did not affect binding of either the rabbit anti-cashew or human cashew allergic IgE binding. Among the treatments, 10 min of plasma processing at flow rate of 30 mL·min−1 of synthetic air followed by 20 min at flow rate 5.8 mL·min−1 had the least effect on nut composition as a whole
