Author(s): Sharkawy, Asma ; Casimiro, Filipa M. ; Barreiro, M.F. ; Rodrigues, Alírio
Date: 2020
Persistent ID: http://hdl.handle.net/10198/22318
Origin: Biblioteca Digital do IPB
Project/scholarship: info:eu-repo/grantAgreement/FCT/POR_NORTE/PD%2FBD%2F135085%2F2017/PT;
Subject(s): Biodegradable; Chitosan/gum Arabic nanoparticles; Photostability; Pickering emulsions; Resveratrol; Topical delivery
The surfactant-free nature and higher stability of Pickering emulsions make them preferable solutions over conventional emulsions for skin applications. In this work, Pickering emulsions stabilized by chitosan/gum Arabic (CH/GA) nanoparticles were tested as vehicles for trans-resveratrol topical delivery. Skin absorption was examined ex vivo using Franz diffusion cells and porcine skin. Pickering emulsions allowed higher cutaneous retention and lower permeation of resveratrol, in comparison with a control solution based on a 20% v/v ethanol. The total amount of resveratrol retained in the skin, 24 h after the application, was 11.60% and 10.82% of the applied dose for the tested Pickering emulsion-based formulations prepared with 0.5% and 1.5%w/v CH/GA nanoparticles, respectively. In contrast, resveratrol skin retention from the control solution was only 2.86%. Confocal laser scanning microscopy revealed enhanced skin deposition of Nile Red to deeper layers from the Pickering emulsionbased formulations. Moreover, Pickering emulsions led to trans-resveratrol photostability increase, as measured after exposure to UV for 4 h. These results show that the CH/GA Pickering emulsions are promising solutions for the topical delivery of trans-resveratrol and have the potential to be used as green cosmetic products.
This work was supported by Associate Laboratory LSRE-LCM - UID/ EQU/50020/2019 - funded by national funds through FCT/MCTES (PIDDAC), and Foundation for Science and Technology (FCT, Portugal); and CIMO (UID/AGR/00690/2019) through FEDER under Program PT2020. The authors acknowledge the technical support provided by the Instituto de Investigação e Inovação (i3s) and CEMUP, University of Porto. The authors are thankful to Dr. Patricia Costa for providing the skin samples, and Tasneem Sharkawi for language editing. Asma Sharkawy acknowledges the financial support from the FCT doctoral grant (PD/BD/135085/2017).
