Author(s): Aguiar, Tatiana Quinta ; Dinis, Cláudia ; Domingues, Lucília
Date: 2014
Persistent ID: http://hdl.handle.net/1822/31659
Origin: RepositóriUM - Universidade do Minho
Project/scholarship: info:eu-repo/grantAgreement/FCT/5876-PPCDTI/126270/PT ;
Subject(s): Ashbya gossypii; Cre-loxP system; Gene targeting; Marker recycling; Recombinase expression; Science & Technology
The filamentous ascomycete Ashbya gossypii is amenable to genetic manipulation and is an excellent model system for studying eukaryotic cell biology. However, the number of selection markers in current use for both targeted gene integration and disruption in this fungus are very limited. Therefore, the Cre-loxP recombination system was adapted for use in A. gossypii and its effectiveness in recycling marker genes was demonstrated by constructing both single and double deleted Agura3 and Agade1 auxotrophic strains free of exogenous markers. In spite of its wide use, this is the first report in which the Cre-loxP system was applied to A. gossypii, opening new perspectives for targeted engineering of this fungus with several promising biotechnological applications.
This work was supported by Fundacao para a Ciencia e a Tecnologia, Portugal, through Project AshByofactory (PTDC/EBB-EBI/101985/2008 - FCOMP-01-0124-FEDER-009701), Project RECI/BBB-EBI/0179/2012 - FCOMP-01-0124-FEDER-027462, Strategic Project PEst-OE/EQB/LA0023/2013, Project BioInd (NORTE-07-0124-FEDER-000028) co-funded by the Programa Operacional Regional do Norte (ON.2 - O Novo Norte), QREN, FEDER, and MIT-Portugal Program PhD Grant SFRH/BD/39112/2007 to Tatiana Q. Aguiar.
