Detalhes do Documento

Antibacterial bioadhesive layer-by-layer coatings for orthopedic applications

Autor(es): Carvalho, A. L. cv logo 1 ; Vale, A. C. cv logo 2 ; Sousa, M. P. cv logo 3 ; Barbosa, A. M. cv logo 4 ; Torrado, Egídio cv logo 5 ; Mano, J. F. cv logo 6 ; Alves, N. M. cv logo 7

Data: 2016

Identificador Persistente: http://hdl.handle.net/1822/43830

Origem: RepositóriUM - Universidade do Minho

Projeto/bolsa: FCT/COMPETE/133668

Assunto(s): Adsorption; Bioactive glass Nanoparticles; Chitosan; Cross-linking; Fabrication; Hyaluronic-acid; Mechanics; Mussel-adhesive; Silver nanoparticles; Surface; Science & Technology


Descrição
In this study, thin LbL films were produced by combining the adhesive properties of the hyaluronic acidâ dopamine conjugate with the bioactivity and bactericidal properties of silver doped bioactive glass nanoparticles. The build-up of these films was investigated by quartz crystal microbalance with dissipation monitoring. LbL coatings were then constructed on a glass substrate for further characterization. We found that these antimicrobial bioinspired films display enhanced adhesive strength. In vitro bioactivity tests were performed by immersing them in simulated body fluid solution for 14 days where the constructed films promoted the formation of a bone-like apatite layer. From microbiological assays, it was found that coatings containing silver doped nanoparticles exhibited a remarkable antibacterial effect against Staphylococcus aureus and Escherichia coli cultures. Finally, in vitro cellular behavior tests showed enhanced cell adhesion, proliferation and viability for these antibacterial bioadhesive films. Therefore, the constructed thin films showed promising properties and evidenced great potential to be used as coatings for orthopedic implants.The authors acknowledge the Portuguese Foundation for Science and Technology (FCT) and the European program FEDER/COMPETE for the financial support through project BioSeaGlue: EXPL/CTM-BIO/0646/2013 (FCOMP-01-0124-FEDER041105). This work was co-funded by ‘‘Programa Operacional Regional do Norte’’ (ON.2-O Novo Norte) under the ‘‘Quadro de Referência Estratégico Nacional’’ (QREN), through the ‘‘Fundo Europeu de Desenvolvimento Regional’’ (FEDER). E. T. also thanks the FCT investigator grant (IF/01390/2014).
Tipo de Documento Artigo
Idioma Inglês
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