Author(s):
Amorim, Cláudia ; Silvério, Sara C. ; Rodrigues, L. R.
Date: 2019
Persistent ID: http://hdl.handle.net/1822/55609
Origin: RepositóriUM - Universidade do Minho
Project/scholarship:
info:eu-repo/grantAgreement/FCT/5876/147337/PT;
info:eu-repo/grantAgreement/FCT/5876-PPCDTI/126270/PT
;
info:eu-repo/grantAgreement/FCT/COMPETE/126270/PT;
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F88584%2F2012/PT;
Subject(s): Agro-residues; Prebiotic; Xylooligosaccharides; Brewers spent grain; Trichoderma; Xxylooligosaccharides; Science & Technology
Description
Supplementary data associated with this article can be found, in the online version, at https://doi.org/10.1016/j.foodchem.2018.07.080.
Xylooligosaccharides (XOS) are prebiotic nutraceuticals that can be sourced from lignocellulosic biomass, such as agro-residues. This study reports for the first time an optimization study of XOS production from agro-residues by direct fermentation using two Trichoderma species. A total of 13 residues were evaluated as potential substrates for single-step production. The best results were found for Trichoderma reesei using brewers spent grain (BSG) as substrate. Under optimal conditions (3 days, pH 7.0, 30°C and 20 g/L of BSG), a production yield of 38.3 ± 1.8 mg/g (xylose equivalents/g of BSG) was achieved. The obtained oligosaccharides were identified as arabino-xylooligosacharides (AXOS) with degree of polymerization from 2 to 5. One-step fermentation proved to be a promising strategy for AXOS production from BSG, presenting a performance comparable with the use of commercial enzymes. This study provides new insights towards the bioprocess integration, enabling further developments of low-cost bioprocesses for the production of these valuable compounds.
This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER006684) and under the scope of the Project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462) and the Project MultiBiorefinery – Multi purpose strategies for broadband agro forest and fisheries by products valorisation: a step forward for a truly integrated biorefinery (POCI-01-0145-FEDER-016403) the Project Lignozymes (POCI-010145-FEDER-029773) and the Project FoSynBio (POCI-01-0145FEDER-029549). The authors also acknowledge financial support from BioTecNorte (NORTE-01-0145-FEDER-000004) Project BioInd – Biotechnology and Bioengineering for improved Industrial and AgroFood processes(NORTE-07–0124-FEDER-000028) funded by the European Regional Development Fund under the scope of Norte2020 Programa Operacional Regional do Norte. CA and SCS acknowledge their grants (PD/BD/105870/2014 SFRH/BPD/88584/2012) from FCT.
info:eu-repo/semantics/publishedVersion
