Author(s):
Costa, Ana Rita ; Milho, Catarina ; Azeredo, Joana ; Pires, Diana Priscila Penso
Date: 2018
Persistent ID: http://hdl.handle.net/1822/56504
Origin: RepositóriUM - Universidade do Minho
Project/scholarship:
info:eu-repo/grantAgreement/FCT/5876/147337/PT;
info:eu-repo/grantAgreement/FCT/5876-PPCDTI/126270/PT
;
info:eu-repo/grantAgreement/FCT/COMPETE/126270/PT;
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F94648%2F2013/PT;
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F94434%2F2013/PT;
Subject(s): Bacteriophage; Bacteriophage engineering; YAC; BRED
Description
Recent advances in the synthetic biology field have enabled the development of new molecular biology techniques used to build specialized bacteriophages with new functionalities. Bacteriophages have been engineered towards a wide range of applications including pathogen control and detection, targeted drug delivery, or even assembly of new materials. In this chapter, two strategies that have been successfully used to genetically engineer bacteriophage genomes are addressed: a yeast-based platform and bacteriophage recombineering of electroporated DNA.
This work was supported by the Portuguese Foundation for Scienceand Technology (FCT)under the scope ofthe project PTDC/ BBB-BSS/6471/2014, the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145FEDER-006684), and under the scope of the Project RECI/ BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462). This work was also supported by BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte. Ana Rita Costa, Catarina Milho and Diana Priscila Pires acknowledge FCT for the grants SFRH/BPD/94648/2013, SFRH/BD/94434/2013 and SFRH/BPD/116187/2016, respectively.
info:eu-repo/semantics/publishedVersion
