Document details

hCES2: studies on the role of glycosylation in activity

Author(s): Alves, Márcia Maria da Silva

Date: 2013

Persistent ID: http://hdl.handle.net/10362/10896

Origin: Repositório Institucional da UNL

Subject(s): Carboxylesterase; Glycosylation; Deglycosylation; PNGase F; Tunicamycin; Site directed mutagenesis


Description

Human carboxylesterase 2 (hCES2) is becoming more relevant due to its role in the hydrolysis of several drugs, pro-drugs and xenobiotics. However, its three dimensional structuture is yet to be resolved, and many of its properties are still unclear. In this work, the impact of glycosylation in the enzymatic activity of an hCES2 variant, hCES2-10xHis, was evaluated. To achieve this objective, different methods were used, such as enzymatic deglycosylation and glycosylation inhibition through specific inhibitors or plasmid mutation. It was concluded that glycosylation is necessary during hCES2 synthesis to maintain protein activity, but the presence of the glycans is not essential after the protein is completely matured.

Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina

Document Type Master thesis
Language English
Advisor(s) Simplício, Ana
Contributor(s) Alves, Márcia Maria da Silva
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