Document details

On the road of sequencing the genomes: Past, Present and Future

Author(s): Flores, Alcino Orfeu

Date: 2015

Persistent ID: http://hdl.handle.net/10362/20502

Origin: Repositório Institucional da UNL

Subject(s): Molecular Genetics; Molecular Genetics; Molecular Genetics


Description

Desulfovibrio gigas is a model organism of sulfate-reducing bacteria of which energy metabolism and stress response have been extensively studied. The complete genomic context of this organism was however not yet available. This thesis is about the sequencing of the D. gigas genome and provides insights into the integrated network of energy conserving complexes and structures present in this bacterium. The work presented describes the technological processes used to sequence the genome. Chapter I is an overview of its most recent advances in bacterial genome sequencing, since the first genome published of Haemophilus influenzae till present; Chapter II is mostly an overview of the 4 different technologies that were necessary to finalize this genome sequencing project: shotgun sequencing through Sanger method and massive DNA sequencing from 3 different next generation sequencing (NGS) platforms: 454 (Roche);(...)

Document Type Doctoral thesis
Language English
Advisor(s) Pousada, Claudina Rodrigues
Contributor(s) Flores, Alcino Orfeu
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