Quercus suber MYB1 transcription factor - an in vivo approach for the elucidation of its regulatory networks

• Author(s): Capote, Tiago Costa
• Date: 2018
• Persistent ID: http://hdl.handle.net/10400.5/15827
• Origin: Repositório da UTL
• Subject(s): QsMYB1; R2-R3; transcription factor; ChIP-Seq; somatic embryogenesis; transcriptome
• Document Type: Doctoral thesis
• Language: English
• Advisor(s): Cecílio, Leonor Morais; Gonçalves, Sónia Cláudia Morgado
• Contributor(s): Repositório da Universidade de Lisboa

Description

Doutoramento em Biologia - Instituto Superior de Agronomia - UL

The study of transcription factors (TFs) can reveal important players behind a specific developmental process. The identification of TFs DNA-targets on a genome-wide scale provides fundamental information not only on how the expression of the targeted genes are regulated but also about the molecular mechanisms behind this process. QsMYB1 is a R2R3-MYB transcription factor related to secondary growth and cork development in Quercus suber. Given the importance of cork quality for the cork-based industry, the study of genes involved in cork formation can provide important insights. In this context, the objective of this PhD thesis was to further study QsMYB1 gene, by identifying its DNA targets on the cork oak genome, in order to elucidate the gene regulatory mechanism in which QsMYB1 exerts its function