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Pesquisa de fitoplasmas das pomóideas e prunóideas em Portugal

Author(s): Marques, Ana Isabel Ambrósio

Date: 2011

Persistent ID:

Origin: Repositório da UTL

Subject(s): phytoplasmas; 16SrX group; psyllids; nested-PCR; real-time PCR; Candidatus; phytoplasma pyri


Phytoplasmas are unicellular organisms, prokaryotes, cell wall-less that cause diseases in hundreds vegetable species. This study is mainly about the prospection of “Candidatus” phytoplasma pyri, “Candidatus” phytoplasma mali and “Candidatus” phytoplasma prunorum, etiologic agents of PD, AP e ESFY, vectored by psyllids Cacopsylla pyri, Cacopsylla picta e Cacopsylla pruni, respectively. These three phytoplasmas belong to AP or 16SrX group, considered quarantine organisms (A2 list, EPPO). In this study, two DNA extraction methods as well as two identification methods using PCR were compared. The two extraction methods differ in lyses buffer which seems to be crucial to obtain high quality DNA. The most used identification method until recently is nested-PCR but as it is a time consuming and expensive method, it’s being replaced by realtime PCR a quicker, more viable and less expensive one, as it was conf irmed in this study. Plant and insect samples from pome and prunus fruit trees in Oeste area were analysed. With major importance lies the fact that, for the first time, we report the presence of “Candidatus” phytoplasma pyri in insect and plant samples.

Mestrado em Engenharia Agronómica - Protecção das Plantas - Instituto Superior de Agronomia

Document Type Master thesis
Language Portuguese
Advisor(s) Sousa, Esmeraldina Agostinho Nascimento; Mexia, António Maria Marques
Contributor(s) Marques, Ana Isabel Ambrósio
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