Autor(es): Duarte, Cristina ; Gudiña, Eduardo J. ; Lima, Cristóvão F. ; Rodrigues, L. R.
Data: 2014
Identificador Persistente: http://hdl.handle.net/1822/31729
Origem: RepositóriUM - Universidade do Minho
Projeto/bolsa: info:eu-repo/grantAgreement/FCT/5876-PPCDTI/126270/PT ;
Assunto(s): Surfactin lipopeptide; Lactobacillus paracasei glycoprotein; Cell viability; Cell cycle; Breast cancer; Science & Technology
Biosurfactants are molecules with surface activity produced by microorganisms that can be used in many biomedical applications. The anti-tumour potential of these molecules is being studied, although results are still scarce and few data are available regarding the mechanisms underlying such activity. In this work, the anti-tumour activity of a surfactin produced by Bacillus subtilis 573 and a glycoprotein (BioEG) produced by Lactobacillus paracasei subsp. paracasei A20 was evaluated. Both biosurfactants were tested against two breast cancer cell lines, T47D and MDA-MB-231, and a non-tumour fibroblast cell line (MC-3T3-E1), specifically regarding cell viability and proliferation. Surfactin was found to decrease viability of both breast cancer cell lines studied. A 24h exposure to 0.05gl-1 surfactin led to inhibition of cell proliferation as shown by cell cycle arrest at G1 phase. Similarly, exposure of cells to 0.15gl-1 BioEG for 48h decreased cancer cells' viability, without affecting normal fibroblasts. Moreover, BioEG induced the cell cycle arrest at G1 for both breast cancer cell lines. The biosurfactant BioEG was shown to be more active than surfactin against the studied breast cancer cells. The results gathered in this work are very promising regarding the biosurfactants potential for breast cancer treatment and encourage further work with the BioEG glycoprotein.
The authors acknowledge the financial support from the Strategic Project PEst-OE/EQB/LA0023/2013 and the project ref. RECI/BBB-EBI/0179/2012 (project number FCOMP-01-0124-FEDER-027462) funded by Fundacao para a Ciencia e a Tecnologia. Moreover, the authors acknowledge IPATIMUP for kindly providing the breast cancer cell lines used in this work.
