Project/scholarship details

  • Funder

    FCT - Fundação para a Ciência e a Tecnologia, I.P.

  • Funder's country

    Portugal

  • Funding program

    5876-PPCDTI

  • Funding amount

    164,000.00 €

  • Start date

    2010-04-01

  • End date

    2013-09-30

Documents

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Physiological characterization of Ashbya gossypii and strain development for re...

Ribeiro, Orquídea

Tese de doutoramento em Engenharia Quimica e Biológica; Ashbya gossypii is a phytopathogenic hemiascomycete belonging to the Saccharomycetaceae family. This fungus has attracted attention because of its natural ability to produce riboflavin (vitamin B2) as a detoxifying and protective mechanism being used at an industrial level as a biotechnological important producer of riboflavin. The genome sequence of this ...

Date: 2012   |   Origin: RepositóriUM - Universidade do Minho
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Engineering yeast tolerance to inhibitory lignocellulosic biomass

Cunha, Joana Filipa Torres Pinheiro; Aguiar, Tatiana Quinta; Mendes, D.; Pereira, Francisco B.; Domingues, Lucília

In recent years the necessity for biotechnological manufacturing based on lignocellulosic feedstocks has become evident. However, the pre-treatment step in the production of lignocellulosic bioethanol leads to the accumulation of inhibitory byproducts. Robust second generation bioethanol processes require microorganisms able to ferment these inhibitory lignocellulosic hydrolysates. Significant progress has been...

Date: 2013   |   Origin: RepositóriUM - Universidade do Minho
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Molecular and functional characterization of an invertase secreted by Ashbya go...

Aguiar, Tatiana Quinta; Dinis, Cláudia; Magalhães, Frederico; Oliveira, Carla Cristina Marques de; Wiebe, Marilyn G.; Penttilä, Merja

The repertoire of hydrolytic enzymes natively secreted by the filamentous fungus Ashbya (Eremothecium) gossypii has been poorly explored. Here, an invertase secreted by this flavinogenic fungus was for the first time molecularly and functionally characterized. Invertase activity was detected in A. gossypii culture supernatants and cell-associated fractions. Extracellular invertase migrated in a native polyacryl...

Date: 2014   |   Origin: RepositóriUM - Universidade do Minho
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Investigation of protein secretion and secretion stress in Ashbya gossypii

Aguiar, Tatiana Quinta; Ribeiro, Orquídea; Arvas, Mikko; Wiebe, Marilyn G.; Penttilä, Merja; Domingues, Lucília

Background: Ashbya gossypii is a filamentous Saccharomycete used for the industrial production of riboflavin that has been recently explored as a host system for recombinant protein production. To gain insight into the protein secretory pathway of this biotechnologically relevant fungus, we undertook genome-wide analyses to explore its secretome and its transcriptional responses to protein secretion stress. Res...

Date: 2014   |   Origin: RepositóriUM - Universidade do Minho
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Genome-wide metabolic re-annotation of Ashbya gossypii: new insights into its m...

Gomes, Daniel Gonçalves; Aguiar, Tatiana Quinta; Dias, Oscar; Ferreira, Eugénio C.; Domingues, Lucília; Rocha, I.

BACKGROUND:Ashbya gossypii is an industrially relevant microorganism traditionally used for riboflavin production. Despite the high gene homology and gene order conservation comparatively with Saccharomyces cerevisiae, it presents a lower level of genomic complexity. Its type of growth, placing it among filamentous fungi, questions how close it really is from the budding yeast, namely in terms of metabolism, th...

Date: 2014   |   Origin: RepositóriUM - Universidade do Minho
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