Project/scholarship details
- Project/scholarship ID
127180
- Reference
PTDC/CTM-BIO/1814/2012
- FundRef URI
- Funder
FCT - Fundação para a Ciência e a Tecnologia, I.P.
- Funder's country
Portugal
- Funding program
5876-PPCDTI
- Funding amount
199,948.00 €
- Start date
2013-01-01
- End date
2015-03-31
Documents
Superhydrophobic chips for cell spheroids high-throughput generation and drug s...
Oliveira, Mariana B.; Neto, Ana I.; Correia, Clara R.; Rial-Hermida, Maria Isabel; Álvarez-Lorenzo, Carmen; Mano, J. F.We suggest the use of biomimetic superhydrophobic patterned chips produced by a bench-top methodology as low-cost and waste-free platforms for the production of arrays of cell spheroids/microtissues by the hanging drop methodology. Cell spheroids have a wide range of applications in biotechnology fields. For drug screening, they allow studying 3D models in structures resembling real living tissues/tumors. In ti...
Enhanced cell affinity of chitosan membranes mediated by superficial cross-link...
Rodríguez-Velázquez, Eustolia; Silva, Maite; Taboada, Pablo; Mano, J. F.; Suárez-Quintanilla, D.; Alatorre-Meda, ManuelIt is well accepted that the surface modification of biomaterials can improve their biocompatibility. In this context, techniques like ion etching, plasma-mediated chemical functionalization, electrospinning, and contact microprinting have successfully been employed to promote the cell adhesion and proliferation of chitosan (CH) substrates. However, they prove to be time-consuming, highly dependent on environme...
Biomimetic miniaturized platform able to sustain arrays of liquid droplets for ...
Neto, Ana I.; Correia, Clara R.; Custódio, Catarina A.; Mano, J. F.The development of high-throughput and combinatorial technologies is helping to speed up research that is applicable in many areas of chemistry, engineering, and biology. A new model is proposed for flat devices for the high-throughput screening of accelerated evaluations of multiplexed processes and reactions taking place in aqueous-based environments. Superhydrophobic (SH) biomimetic surfaces based on the so-...
Molecular interactions driving the layer-by-layer assembly of multilayers
Borges, J.; Mano, J. F.This article presents an overview of the different types of intermolecular interactions behind the fabrication of multilayer assemblies using the layer-by-layer (LbL) assembly approach. It comments on the potential impact of each type of intermolecular interaction and materials assembled through them on the development of advanced functional systems or devices for several emerging applications. The discussion b...
Sequential ionic and thermogelation of chitosan spherical hydrogels prepared us...
Lima, A. C.; Correia, Clara R.; Oliveira, Mariana B.; Mano, J. F.Chitosan is soluble in acidic media, which makes it incompatible for the encapsulation of cells and pH-sensitive molecules. In this work, a mild chitosan-based system with two sequential gelation steps is proposed, where the model drug dexamethasone and L929 cells are immobilized inside hydrogel beads. Superhydrophobic surfaces were used to produce the spherical hydrogel particles that provided favorable condit...
Biocompatible polymeric microparticles produced by a simple biomimetic approach
Costa, A. M. S.; Alatorre-Meda, Manuel; Oliveira, N. M.; Mano, J. F.The use of superhydrophobic surfaces to produce polymeric particles proves to be biologically friendly since it entails the pipetting and subsequent cross-linking of polymeric solutions under mild experimental conditions. Moreover, it renders encapsulation efficiencies of ∼100%. However, the obtained particles are 1 to 2 mm in size, hindering to a large extent their application in clinical trials. Improving on ...
Free and copolymerized gamma-cyclodextrins regulate performance of dexamethason...
Lima, A. C.; Puga, Ana M.; Mano, J. F.; Concheiro, Angel; Alvarez-Lorenzo, CarmenPolymeric particles acting as source of biological cues to promote tissue regeneration are currently an interesting topic in bone tissue engineering research. In this study, microspheres of dextran-methacrylated (dextran-MA) and γ-cyclodextrins (γ-CD) for the delivery of osteogenic agents were prepared by means of photopolymerization on biomimetic superhydrophobic surfaces. The effects of the incorporation of t...
Development of natural-based hydrogel particles using a biomimetic methodology
Lima, Ana Catarina CardosoTese de doutoramento em Bioengenharia; Superhydrophobic (SH) surfaces have been greatly explored in the biomedical field. Such surfaces are inspired by the repellent properties of natural structures. The most well known example is the lotus leaf, which has the capacity to repeal the water droplets due to the presence of micro/nano topographical features and the low surface energy. One particular application of ...
A novel hanging spherical drop system for the generation of cellular spheroids ...
Neto, A. I.; Correia, C. R.; Oliveira, M. B.; Rial-Hermida, M. I.; Álvarez-Lorenzo, C.; Reis, R. L.; Mano, J. F.We propose a novel hanging spherical drop system for anchoring arrays of droplets of cell suspension based on the use of biomimetic superhydrophobic flat substrates, with controlled positional adhesion and minimum contact with a solid substrate. By facing down the platform, it was possible to generate independent spheroid bodies in a high throughput manner, in order to mimic in vivo tumour models on the lab-on-...
Highly robust chitosan hydrogels via a fast, simple and biocompatible dual cros...
Costa, A. M. S.; Mano, J. F.Load-bearing soft tissues such as cartilage, blood vessels and muscles are able to withstand a remarkable compressive stress of several MPa without fracturing. Interestingly, most of these structural tissues are mainly composed of water and in this regard, hydrogels, as highly hydrated 3D-crosslinked polymeric networks, constitute a promising class of materials to repair lesions on these tissues. Although sever...
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