Project/scholarship details


Mycotoxins are secondary metabolites produced by molds, which are toxic to animals in small amounts and ubiquitous in many agricultural commodities. Since they have been recognized as a potential threat to human and animal health, many countries have regulations to control their presence in food and feed. The occurrence of mycotoxins in fields is inevitable under certain environmental conditions. Therefore, it is recommended that their presence should be reduced as much as technologically possible at the post-harvest level. The present methods and technologies are not always effective on that, and some may even significantly change the nutritional quality of food and feed. Therefore, researchers are still looking for better solutions. Presently, the biological approaches hold a leading role among sought strategies. Their main focus has been to find microorganisms and/or enzymes that can transform or biodegrade mycotoxins into non-toxic compounds. Lactic acid bacteria (LAB) are considered health beneficial microorganisms because they are traditionally used in the production of fermented food and feed products, and because they have several well known probiotic properties. Nevertheless, more recently they also began to be known for their ability to detoxify mycotoxins. This unusual LAB characteristic is strain specific and involves adsorption and metabolization mechanisms. Presently, active strains are very rare and the involved mechanisms are not yet fully understood and clarified by researchers. They may be used as probiotic for animal nutrition, as inoculants for silages, as biocontrol agents in crop fields, as starter cultures for fermented products, and/or as starter cultures for wine malolactic fermentations. The present project intends to explore the potential of LAB to detoxify mycotoxins in food products and to elucidate some fundamental research questions on this subject.

  • Funder

    FCT - Fundação para a Ciência e a Tecnologia, I.P.

  • Funder's country

    Portugal

  • Funding program

    5876-PPCDTI

  • Funding amount

    96,054.00 €

  • Start date

    2013-05-01

  • End date

    2015-07-31

Documents


Analytical evaluation of fining treatments for white wines contaminated by Ochr...

Carvalho, Filipa; Inês, António; Nunes, Fernando M.; Filipe-Ribeiro, Luís; Abrunhosa, Luís; Cosme, Fernanda

Mycotoxins are toxic secondary metabolites produced by certain molds. Ochratoxin A (OTA) is one of the most relevant. Its chemical structure is a dihydro-isocoumarin connected at the 7-carboxy group to a molecule of L--phenylalanine via an amide bond. OTA in wine is a risk to consumer health [1]. According to the Regulation No. 123/2005 of the European Commission, the maximum limit for OTA in wine is 2 µg/kg [2...


Use of Pediococcus parvulus to remove ochratoxin A from wine

Guimarães, Ana Cristina Silva Esperança; Pereira, Vânia L.; Inês, António; Abrunhosa, Luís

A ocratoxina A (OTA) é uma micotoxina que pode ser encontrada no café, vinho tinto e frutos secos mas a sua principal fonte são os cereais e os seus derivados. É produzida principalmente por Penicillium verrucosum, Aspergillus westerdijkiae, Aspergillus carbonarius e Aspergillus niger. Estas duas últimas espécies são muito comuns em uvas e são responsáveis pela presença de OTA nos vinhos. Na Europa, o vinho é a...


Biodegradation of ochratoxin A by Pediococcus parvulus isolated from Douro wines

Abrunhosa, Luís; Inês, António; Rodrigues, A. I.; Guimarães, Ana Cristina Silva Esperança; Pereira, Vânia L.; Parpot, Pier; Mendes-Faia, Arlete

Lactic acid bacteria (LAB) are a promising solution to reduce exposure to dietary mycotoxins because of the unique mycotoxin decontaminating characteristic of some LAB. Ochratoxin A (OTA) is one of the most prominent mycotoxins found in agricultural commodities. The present work reports on the ability of Pediococcus parvulus strains that were isolated from Douro wines that spontaneously underwent malolactic fer...


Zearalenone and its derivatives α-Zearalenol and β-Zearalenol decontamination b...

Keller, Luiz; Abrunhosa, Luís; Keller, Kelly; Rosa, Carlos Alberto; Cavaglieri, Lilia; Venâncio, Armando

Zearalenone (ZEA) and its derivatives are mycotoxins with estrogenic effects on mammals. The biotransformation for ZEA in animals involves the formation of two major metabolites, - and -zearalenol (-ZOL and -ZOL), which are subsequently conjugated with glucuronic acid. The capability of Saccharomyces cerevisiae strains isolated from silage to eliminate ZEA and its derivatives -ZOL and -ZOL was investigated as, ...


Influence of different activated carbons on Ochratoxin A decrease in wines

Inês, António; Silva, Davide; Nunes, Fernando M.; Filipe-Ribeiro, Luís; Abrunhosa, Luís; Cosme, Fernanda

The presence of mycotoxins in foodstuff is a matter of concern for food safety. Wines can also be contaminated with these toxicants. Several authors have demonstrated the presence of mycotoxins in wine, especially ochratoxin A (OTA) [1]. As these toxicants can never be completely removed from the food chain, many countries have defined levels in food in order to attend health concerns. The maximum acceptable le...


Micotoxinas no vinho: problemas e soluções

Abrunhosa, Luís

Financiado pelo FEDER através do COMPETE e pela FCT (Ref. FCOMP-01-0124-FEDER-028029 e PTDC/AGR-TEC/3900/2012, respetivamente). Incentivo/EQB/LA0023/2014 do programa ON.2 – O Novo Norte.


Control of Aflatoxigenic fungi and mycotoxins production by lactobacillus species

Guimarães, Ana Cristina Silva Esperança; Santiago, Ana P.; Venâncio, Armando; Teixeira, J. A.; Abrunhosa, Luís

Molds play an important role in spoilage of food products. It is estimated that 5 to 10% of the world foods production is lost due to fungal contamination. Further, certain fungal species produce highly toxic metabolites designated of mycotoxins. Aflatoxins are the most toxics because they are proven carcinogenic. Biopreservation, defined as the control of one organism by another, has received much attention in...


Removal of ochratoxin A from contaminated white and red wines using oenological...

Cosme, Fernanda; Carvalho, Filipa; Nunes, Fernando M.; Filipe-Ribeiro, Luís; Abrunhosa, Luís; Inês, António

Mycotoxins are toxic secondary metabolites produced by certain moulds, being ochratoxin A (OTA) one of the most relevant. Its chemical structure is a dihydro-isocoumarin connected at the 7-carboxy group to a molecule of L--phenylalanine via an amide bond. OTA contamination of wines might be a risk to consumer health, thus requiring treatments to achieve acceptable standards for human consumption [1]. According ...


New bio-strategies for ochratoxin A detoxification using lactic acid bacteria

Maria, José; Laranjo, Vânia; Abrunhosa, Luís; Inês, António

The occurrence of mycotoxigenic moulds such as Aspergillus, Penicillium and Fusarium in food and feed has an important impact on public health, by the appearance of acute and chronic mycotoxicoses in humans and animals, which is more severe in the developing countries due to lack of food security, poverty and malnutrition. This mould contamination also constitutes a major economic problem due the lost of crop p...


Food safety in wine: removal of ochratoxin A in contaminated white wine using c...

Inês, António; Silva, Davide; Carvalho, Filipa; Filipe-Ribeiro, Luís; Nunes, Fernando M.; Abrunhosa, Luís; Cosme, Fernanda

The presence of mycotoxins in foodstuff is a matter of concern for food safety. Mycotoxins are toxic secondary metabolites produced by certain molds, being ochratoxin A (OTA) one of the most relevant. Wines can also be contaminated with these toxicants. Several authors have demonstrated the presence of mycotoxins in wine, especially ochratoxin A (OTA) [1]. Its chemical structure is a dihydro-isocoumarin connect...

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