Project/scholarship details


  • Funder

    FCT - Fundação para a Ciência e a Tecnologia, I.P.

  • Funder's country

    Portugal

  • Funding program

    5876-PPCDTI

  • Funding amount

    338,852.00 €

  • Start date

    2013-01-01

  • End date

    2015-12-31

Documents


A size exclusion HPLC method for evaluating the individual impacts of sugars an...

Dias, L.G.; Sequeira, Cédric Basílio; Veloso, Ana C.A.; Morais, Jorge Sá; Sousa, Mara E.B.C.; Peres, António M.

In this work, the main organic acids (citric, malic and ascorbic acids) and sugars (glucose, fructose and sucrose) present in commercial fruit beverages (fruit carbonated soft-drinks, fruit nectars and fruit juices) were determined. A novel size exclusion high performance liquid chromatography isocratic green method, with ultraviolet and refractive index detectors coupled in series, was developed. This methodol...

Date: 2014   |   Origin: Biblioteca Digital do IPB

Investigation of protein secretion and secretion stress in Ashbya gossypii

Aguiar, Tatiana Quinta; Ribeiro, Orquídea; Arvas, Mikko; Wiebe, Marilyn G.; Penttilä, Merja; Domingues, Lucília

Background: Ashbya gossypii is a filamentous Saccharomycete used for the industrial production of riboflavin that has been recently explored as a host system for recombinant protein production. To gain insight into the protein secretory pathway of this biotechnologically relevant fungus, we undertook genome-wide analyses to explore its secretome and its transcriptional responses to protein secretion stress. Res...


Cre-loxP-based system for removal and reuse of selection markers in Ashbya goss...

Aguiar, Tatiana Quinta; Dinis, Cláudia; Domingues, Lucília

The filamentous ascomycete Ashbya gossypii is amenable to genetic manipulation and is an excellent model system for studying eukaryotic cell biology. However, the number of selection markers in current use for both targeted gene integration and disruption in this fungus are very limited. Therefore, the Cre-loxP recombination system was adapted for use in A. gossypii and its effectiveness in recycling marker gen...


Design of experiments to assess pre-treatment and co-digestion strategies that ...

Oliveira, J. V. M.; Alves, M. M.; Costa, J. C.

A design of experiments was applied to evaluate different strategies to enhance the methane yield of macroalgae Gracilaria vermiculophylla. Biochemical Methane Potential (BMP) of G. vermiculophylla after physical pre-treatment (washing and maceration) reached 481 ± 9 L CH4 kg−1 VS, corresponding to a methane yield of 79 ± 2%. No significant effects were achieved in the BMP after thermochemical pre-treatment, al...


Networking the way towards antimicrobial combination therapies

Jorge, Paula Alexandra Silva; Pereira, Maria Olívia; Lourenço, Anália

The exploration of new antimicrobial combinations is a pressing concern for Clinical Microbiology due to the growing number of resistant strains emerging in healthcare settings and in the general community. Researchers are screening agents with alternative modes of action and interest is rising for the potential of antimicrobial peptides (AMPs). This work presents the first ever network reconstruction of AMP co...


On the independence of hydrogen production from methanogenic suppressor in oliv...

Gonçalves, Marta R.; Costa, J. C.; Pereira, M. A.; Abreu, A. A.; Alves, M. M.

Anaerobic degradation of olive mill wastewater (OMW) at concentrations ranging from 2 to 100 g/L of chemical oxygen demand (COD) was assessed in batch assays. Methane was the main final product obtained for the lower concentrations tested. For 25 g COD/L, H2 was temporarily produced, albeit H2 depletion occurred, likely due to homoacetogenesis, since acetate was formed concomitantly. Hydrogen was produced and a...


A size exclusion HPLC method for evaluating the individual impacts of sugars an...

Dias, Luís G.; Sequeira, Cédric; Veloso, Ana C. A.; Morais, Jorge Sá; Sousa, Mara E. B. C.; Peres, António M.

In this work, the main organic acids (citric, malic and ascorbic acids) and sugars (glucose, fructose and sucrose) present in commercial fruit beverages (fruit carbonated soft-drinks, fruit nectars and fruit juices) were determined. A novel size exclusion high performance liquid chromatography isocratic green method, with ultraviolet and refractive index detectors coupled in series, was developed. This methodol...


Phenotypic profiling of several strains of Pseudomonas aeruginosa : identificat...

Rodrigues, Alícia Vanessa Fontes

Numerous studies have described that bacteria display higher resistance to antimicrobials when growing in biofilm mode of growth, however the phenotypic and virulence factors changes associated with this increased resistance are still poorly understood. The goal of this study was to enlarge the knowledge about the augmented biofilmrelated resistance of Pseudomonas aeruginosa characterizing phenotypically P. aer...


Gardnerella vaginalis outcompetes 29 other bacterial species isolated from BV p...

Alves, Patrícia Maria; Castro, J.; Sousa, Cármen; Cereija, Tatiana Barros Reis; Cerca, Nuno

Despite the worldwide prevalence of bacterial vaginosis (BV), its etiology is still unknown. Although BV has been associated with the presence of biofilm, the ability of BV-associated bacteria to form biofilms is still largely unknown. Here, we isolated 30 BV-associated species and characterized their virulence, using an in vitro biofilm formation model. Our data suggests that Gardnerella vaginalis had the high...


Pseudomonas aeruginosa diversification during infection development in cystic f...

Sousa, A. M.; Pereira, Maria Olívia

Pseudomonas aeruginosa is the most prevalent pathogen of cystic fibrosis (CF) lung disease. Its long persistence in CF airways is associated with sophisticated mechanisms of adaptation, including biofilm formation, resistance to antibiotics, hypermutability and customized pathogenicity in which virulence factors are expressed according the infection stage. CF adaptation is triggered by high selective pressure o...

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