126270
RECI/BBB-EBI/0179/2012
FCT - Fundação para a Ciência e a Tecnologia, I.P.
Portugal
5876-PPCDTI
338,852.00 €
2013-01-01
2015-12-31
In this work, the main organic acids (citric, malic and ascorbic acids) and sugars (glucose, fructose and sucrose) present in commercial fruit beverages (fruit carbonated soft-drinks, fruit nectars and fruit juices) were determined. A novel size exclusion high performance liquid chromatography isocratic green method, with ultraviolet and refractive index detectors coupled in series, was developed. This methodol...
Background: Ashbya gossypii is a filamentous Saccharomycete used for the industrial production of riboflavin that has been recently explored as a host system for recombinant protein production. To gain insight into the protein secretory pathway of this biotechnologically relevant fungus, we undertook genome-wide analyses to explore its secretome and its transcriptional responses to protein secretion stress. Res...
The filamentous ascomycete Ashbya gossypii is amenable to genetic manipulation and is an excellent model system for studying eukaryotic cell biology. However, the number of selection markers in current use for both targeted gene integration and disruption in this fungus are very limited. Therefore, the Cre-loxP recombination system was adapted for use in A. gossypii and its effectiveness in recycling marker gen...
A design of experiments was applied to evaluate different strategies to enhance the methane yield of macroalgae Gracilaria vermiculophylla. Biochemical Methane Potential (BMP) of G. vermiculophylla after physical pre-treatment (washing and maceration) reached 481 ± 9 L CH4 kg−1 VS, corresponding to a methane yield of 79 ± 2%. No significant effects were achieved in the BMP after thermochemical pre-treatment, al...
The exploration of new antimicrobial combinations is a pressing concern for Clinical Microbiology due to the growing number of resistant strains emerging in healthcare settings and in the general community. Researchers are screening agents with alternative modes of action and interest is rising for the potential of antimicrobial peptides (AMPs). This work presents the first ever network reconstruction of AMP co...
Anaerobic degradation of olive mill wastewater (OMW) at concentrations ranging from 2 to 100 g/L of chemical oxygen demand (COD) was assessed in batch assays. Methane was the main final product obtained for the lower concentrations tested. For 25 g COD/L, H2 was temporarily produced, albeit H2 depletion occurred, likely due to homoacetogenesis, since acetate was formed concomitantly. Hydrogen was produced and a...
In this work, the main organic acids (citric, malic and ascorbic acids) and sugars (glucose, fructose and sucrose) present in commercial fruit beverages (fruit carbonated soft-drinks, fruit nectars and fruit juices) were determined. A novel size exclusion high performance liquid chromatography isocratic green method, with ultraviolet and refractive index detectors coupled in series, was developed. This methodol...
Numerous studies have described that bacteria display higher resistance to antimicrobials when growing in biofilm mode of growth, however the phenotypic and virulence factors changes associated with this increased resistance are still poorly understood. The goal of this study was to enlarge the knowledge about the augmented biofilmrelated resistance of Pseudomonas aeruginosa characterizing phenotypically P. aer...
Despite the worldwide prevalence of bacterial vaginosis (BV), its etiology is still unknown. Although BV has been associated with the presence of biofilm, the ability of BV-associated bacteria to form biofilms is still largely unknown. Here, we isolated 30 BV-associated species and characterized their virulence, using an in vitro biofilm formation model. Our data suggests that Gardnerella vaginalis had the high...
Pseudomonas aeruginosa is the most prevalent pathogen of cystic fibrosis (CF) lung disease. Its long persistence in CF airways is associated with sophisticated mechanisms of adaptation, including biofilm formation, resistance to antibiotics, hypermutability and customized pathogenicity in which virulence factors are expressed according the infection stage. CF adaptation is triggered by high selective pressure o...
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