Project/scholarship details


  • Funder

    FCT - Fundação para a Ciência e a Tecnologia, I.P.

  • Funder's country

    Portugal

  • Funding program

    5876-PPCDTI

  • Funding amount

    338,852.00 €

  • Start date

    2013-01-01

  • End date

    2015-12-31

Documents


Heteroresistance to colistin in Klebsiella pneumoniae is triggered by small col...

Silva, A.; Sousa, Ana Margarida; Alves, Diana; Lourenço, Anália; Pereira, Maria Olívia

The emergence of Klebsiella pneumoniae multidrug-resistant strains pave the way to the re-introduction of colistin as a salvage therapy. However, recent planktonic studies have reported several cases of heteroresistance to this antimicrobial agent. The aim of the present work was to gain better understanding about the response of K. pneumoniae biofilms to colistin antibiotherapy and inspect the occurrence of he...


Co-immobilization of Palm and DNase I for the development of an effective anti-...

Alves, D.; Magalhães, Andreia; Grzywacz, Daria; Neubauer, Damian; Kamysz, Wojciech; Pereira, Maria Olívia

Biomaterial-associated infections, in particular, catheter-associated infections (CAI) are a major problem in clinical practice due to their ability to resist antimicrobial treatment and the host immune system. This study aimed to co-immobilize the antimicrobial lipopeptide Palm and the enzyme DNase I to introduce both antimicrobial and anti-adhesive functionalities to polydimethylsiloxane (PDMS) material, usin...


Conversion of Cn-unsaturated into Cn-2-saturated LCFA can occur uncoupled from ...

Cavaleiro, A. J.; Pereira, M. A.; Guedes, Ana P.; Stams, Alfons Johannes Maria; Alves, M. M.; Sousa, Diana Zita Machado

Fat, oils, and grease present in complex wastewater can be readily converted to methane, but the energy potential of these compounds is not always recyclable, due to incomplete degradation of long chain fatty acids (LCFA) released during lipids hydrolysis. Oleate (C18:1) is generally the dominant LCFA in lipid-containing wastewater, and its conversion in anaerobic bioreactors results in palmitate (C16:0) accumu...


Potential of mannan or dextrin nanogels as vaccine carrier/adjuvant systems

Gonçalves, Catarina; Ferreira, S.; Correia, Alexandra L.; Lopes, Célia; Fleming, Carolina E.; Rocha, Eduardo; Vilanova, Manuel; Gama, F. M.

Polymeric nanogels have been sophisticatedly designed promising a new generation of vaccine delivery/adjuvant systems capable of boosting immune response, a strategic priority in vaccine design. Here, nanogels made of mannan or dextrin were evaluated for their potential as carriers/adjuvants in vaccine formulations. Since lymph nodes are preferential target organs for vaccine delivery systems, nanogels were bio...


Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kin...

Sampaio, Liliana M. P.; Padrão, Jorge; Faria, Jorge; Silva, João P.; Silva, Carla J.; Dourado, Fernando; Zille, Andrea

This work studied the physical immobilization of a commercial laccase on bacterial nanocellulose (BNC) aiming to identify the laccase antibacterial properties suitable for wound dressings. Physico-chemical analysis demonstrates that the BNC structure is manly formed by pure crystalline I cellulose. The pH optimum and activation energy of free laccase depends on the substrate employed corresponding to pH 6, 7, 3...


Valorization of eucalyptus wood by glycerol-organosolv pretreatment within the ...

Romaní, Aloia; Ruiz, Héctor A.; Teixeira, J. A.; Domingues, Lucília

The efficient utilization of lignocellulosic biomass and the reduction of production cost are mandatory to attain a cost-effective lignocellulose-to-ethanol process. The selection of suitable pretreatment that allows an effective fractionation of biomass and the use of pretreated material at high-solid loadings on saccharification and fermentation (SSF) processes are considered promising strategies for that pur...


Oxygen transfer rate and pH as major operating parameters of citric acid produc...

Ferreira, P.; Lopes, Marlene; Mota, M.; Belo, Isabel

The amount of citric acid (CA) produced by Yarrowia lipolytica is dependent on the yeast strain and growth conditions such as pH, oxygen availability and medium composition. In this work, an experimental design based on the Taguchi method was applied to evaluate the effect of parameters: pH, carbon/nitrogen (C/N) ratio in the medium, oxygen mass transfer rate (OTR) and salts concentration, on the CA production ...


Influence of the metabolic state on the tolerance of Pichia kudriavzevii to hea...

Mesquita, Vanessa A.; Machado, Manuela D.; Silva, Cristina F.; Soares, Eduardo V.

This work aims to examine the influence of the metabolic state of the yeast Pichia kudriavzevii on the susceptibility to a metals mixture (5mgL1 Cd, 10mgL1 Pb, and 5mgL1 Zn). Cells exposed to the metals mixture in the presence of 25mmolL1 glucose displayed a higher loss of membrane integrity and proliferation capacity, compared to cells incubated in the absence of glucose. The analysis of the effect of individu...


Bacterial cellulose-lactoferrin as an antimicrobial edible packaging

Padrão, Jorge; Gonçalves, S.; Silva, João P.; Sencadas, Vítor João Gomes Silva; Lanceros-Méndez, S.; Pinheiro, A. C.; Vicente, A. A.; Rodrigues, L. R.

Bacterial cellulose (BC) films from two distinct sources (obtained by static culture with Gluconacetobacter xylinus ATCC 53582 (BC1) and from a commercial source (BC2)) were modified by bovine lactoferrin (bLF) adsorption. The functionalized films (BC+bLF) were assessed as edible antimicrobial packaging, for use in direct contact with highly perishable foods, specifically fresh sausage as a model of meat produc...


Boosting dark fermentation with co-cultures of extreme thermophiles for biohyth...

Abreu, A. A.; Tavares, F.; Alves, M. M.; Pereira, M. A.

Proof of principle of biohythane and potential energy production from garden waste (GW) is demonstrated in this study in a two-step process coupling dark fermentation and anaerobic digestion. The synergistic effect of using co-cultures of extreme thermophiles to intensify biohydrogen dark fermentation is demonstrated using xylose, cellobiose and GW. Co-culture of Caldicellulosiruptor saccharolyticus and Thermot...

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