Project/scholarship details


  • Funder

    FCT - Fundação para a Ciência e a Tecnologia, I.P.

  • Funder's country

    Portugal

  • Funding program

    5876-PPCDTI

  • Funding amount

    338,852.00 €

  • Start date

    2013-01-01

  • End date

    2015-12-31

Documents


Co-digestion of Sargassum sp. with glycerol and waste frying oil: optimization ...

Oliveira, João Vítor; Alves, M. M.; Costa, J. C.

A response surface methodology was adopted to assess the optimal conditions for methane production from the macroalgae Sargassum sp. Three variables were tested: % total solids of algae (%TSsargassum); co-substrate concentration (gwaste L-1); and, co-substrate type (glycerol or waste frying oil (WFO)). The Biochemical Methane Potential (BMP) of Sargassum sp. was 300 ± 3 L CH4 kg-1 COD. The co-digestion with gly...


Dietary sugars analysis : quantification of fructooligossacharides during ferme...

Correia, Daniela M.; Dias, Luís G.; Veloso, Ana C. A.; Dias, T.; Rocha, I.; Rodrigues, L. R.; Peres, A. M.

In this work, a simple chromatographic method is proposed and in-house validated for the quantification of total and individual fructooligossacharides (e.g., 1-kestose, nystose, and 1F-fructofuranosylnystose). It was shown that a high-performance liquid chromatography with refractive index detector could be used to monitor the dynamic of fructooligossacharides production via sucrose fermentation using Aspergill...


Effects of biosurfactants on the viability and proliferation of human breast ca...

Duarte, Cristina; Gudiña, Eduardo J.; Lima, Cristóvão F.; Rodrigues, L. R.

Biosurfactants are molecules with surface activity produced by microorganisms that can be used in many biomedical applications. The anti-tumour potential of these molecules is being studied, although results are still scarce and few data are available regarding the mechanisms underlying such activity. In this work, the anti-tumour activity of a surfactin produced by Bacillus subtilis 573 and a glycoprotein (Bio...


Cre-loxP-based system for removal and reuse of selection markers in Ashbya goss...

Aguiar, Tatiana Quinta; Dinis, Cláudia; Domingues, Lucília

The filamentous ascomycete Ashbya gossypii is amenable to genetic manipulation and is an excellent model system for studying eukaryotic cell biology. However, the number of selection markers in current use for both targeted gene integration and disruption in this fungus are very limited. Therefore, the Cre-loxP recombination system was adapted for use in A. gossypii and its effectiveness in recycling marker gen...


A size exclusion HPLC method for evaluating the individual impacts of sugars an...

Dias, L.G.; Sequeira, Cédric Basílio; Veloso, Ana C.A.; Morais, Jorge Sá; Sousa, Mara E.B.C.; Peres, António M.

In this work, the main organic acids (citric, malic and ascorbic acids) and sugars (glucose, fructose and sucrose) present in commercial fruit beverages (fruit carbonated soft-drinks, fruit nectars and fruit juices) were determined. A novel size exclusion high performance liquid chromatography isocratic green method, with ultraviolet and refractive index detectors coupled in series, was developed. This methodol...

Date: 2014   |   Origin: Biblioteca Digital do IPB

A critical evaluation of methods for the reconstruction of tissue-specific models

Correia, S.; Rocha, Miguel

Under the framework of constraint based modeling, genome-scale metabolic models (GSMMs) have been used for several tasks, such as metabolic engineering and phenotype prediction. More recently, their application in health related research has spanned drug discovery, biomarker identification and host-pathogen interactions, targeting diseases such as cancer, Alzheimer, obesity or diabetes. In the last years, the d...


Aromatic amines sources, environmental impact and remediation

Pereira, Luciana; Mondal, P. K.; Alves, M. M.

Aromatic amines are widely used industrial chemicals as their major sources in the environment include several chemical industry sectors such as oil refining, synthetic polymers, dyes, adhesives, rubbers, perfume, pharmaceuticals, pesticides and explosives. They result also from diesel exhaust, combustion of wood chips and rubber and tobacco smoke. Some types of aromatic amines are generated during cooking, spe...


MorphoCol: an ontology-based knowledgebase for the characterisation of clinical...

Sousa, A. M.; Pereira, Maria Olívia; Lourenço, Anália

Background One of the major concerns of the biomedical community is the increasing prevalence of antimicrobial resistant microorganisms. Recent findings show that the diversification of colony morphology may be indicative of the expression of virulence factors and increased resistance to antibiotic therapeutics. To transform these findings, and upcoming results, into a valuable clinical decision making tool, co...


Biohythane production from marine macroalgae Sargassum sp. coupling dark fermen...

Costa, J. C.; Oliveira, J. V.; Pereira, M. A.; Alves, M. M.; Abreu, A. A.

Potential biohythane production from Sargassum sp. was evaluated in a two stage process. In the first stage, hydrogen dark fermentation was performed by Caldicellulosiruptor saccharolyticus. Sargassum sp. concentrations (VS) of 2.5, 4.9 and 7.4 g L−1 and initial inoculum concentrations (CDW) of 0.04 and 0.09 g L−1 of C. saccharolyticus were used in substrate/inoculum ratios ranging from 28 to 123. The end produ...


Activity of carvacrol against Coagulase-negative Staphylococci biofilm related ...

Lima, Cláudia Sofia Afonso de

Coagulase-negative staphylococci (CoNS) are frequently found in healthy human skin and mucosae, and hence, have long been considered non-pathogenic bacteria. However, in recent years, they have been recognized as important etiological agents of healthcareassociated infections, being particularly associated with patients with indwelling medical devices. Despite their importance as nosocomial pathogens, very litt...

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