Project/scholarship details
- Project/scholarship ID
126270
- Reference
RECI/BBB-EBI/0179/2012
- FundRef URI
- Funder
FCT - Fundação para a Ciência e a Tecnologia, I.P.
- Funder's country
Portugal
- Funding program
5876-PPCDTI
- Funding amount
338,852.00 €
- Start date
2013-01-01
- End date
2015-12-31
Documents
Electrochemical aptasensor array for multiple detection of human osteopontin
Meirinho, S.; Dias, L. G.; Peres, A. M.; Rodrigues, L. R.Electrochemical aptasensor arrays have been recently used to detect several proteins reported as disease biomarkers, mainly due to their known advantages, that include high affinity, sensitivity, specificity and low costs, among others. This study describes the development of a label-free electrochemical multi-aptasensor array, for the simultaneous detection of human osteopontin (OPN), using two specific aptame...
Control of Salmonella Enteritidis on food contact surfaces with bacteriophage P...
Milho, Catarina; Silva, Maria Daniela; Melo, Luís Daniel Rodrigues; Santos, Sílvio Roberto Branco; Azeredo, Joana; Sillankorva, SannaSalmonella is one of the worldwide leading foodborne pathogens responsible for illnesses and hospitalizations, and its capacity to form biofilms is one of its many virulence factors. This work evaluated (bacterio)phage control of adhered and biofilm cells of Salmonella Enteritidis on three different substrata at refrigerated and room temperatures, and also a preventive approach in poultry skin. PVP-SE2 phage wa...
Oil and hydrocarbon-producing bacteria
Castro, Rita; Cavaleiro, Ana Júlia; Pereira, M. A.Bacteria are recognized as a sustainable source of renewable feedstocks for production of biofuels and other chemicals. Triacylglycerols and wax esters, with potential applications similar to the ones derived from plants, can be produced by several groups of bacteria using inexpensive carbon sources, such as organic residues from industry or municipal sources. Also, aliphatic hydrocarbons, which are the main co...
Biofilm applications of bacteriophages
Milho, Catarina; Silva, Maria Daniela; Sillankorva, SannaIndustrial settings, the food processing industry in particular, have strict sanitation procedures to minimize food product contamination and guarantee that their products are safe to be consumed. Sanitation procedures are routinely used, yet still pathogens are recovered from foods and processing surfaces that may eventually cause foodborne illnesses. Microorganisms have a natural tendency to attach to surface...
Comfort and infection control of chitosan-impregnated cotton gauze as wound dre...
Souza, Jefferson M.; Henriques, Mariana; Teixeira, Maria Pilar Araújo; Fernandes, Margarida Maria Macedo; Fangueiro, Raúl; Zille, AndreaThe aim of this study was to evaluate the thermo-physiological comfort properties of surgical cotton gauze coated with chitosan (CH) and its effectiveness for the prevention of bacterial colonization. Gauze was coated with CH at mass fractions of 0.50, 0.25, 0.125, 0.10, 0.063 wt% and the friction, flexibility, thermal, moisture management and mechanical properties were evaluated. The best performing gauze in t...
Hybrid gels: Influence of oleogel/hydrogel ratio on rheological and textural pr...
Martins, Artur J.; Silva, Pedro Miguel Peixoto; Maciel, José Filipe Gonçalves; Pastrana, Lorenzo M.; Cunha, Rosiane LopesSupplementary data to this article can be found online at https:// doi.org/10.1016/j.foodres.2018.10.019.; Hybrid gels can be used for controlled delivery of bioactives and for textural and rheological modification of foods. In this regard the hydrogel:oleogel ratio and gel development methodologies showed to be the aspects that influence most of their properties. The present study shows how different fractions...
Antimicrobial assessment of phage therapy using a porcine model of biofilm infe...
Milho, Catarina; Andrade, M.; Boas, Diana Patrícia Andrade Vilas; Alves, Diana; Sillankorva, SannaAntibiotic resistant bacterial communities persist in many types of wounds, chronic wounds in particular, in the form of biofilms. Biofilm formation is a major cause of severe infections and the main reason for a negative treatment outcome and slow healing progression. Chronic wounds are a silent epidemic essentially affecting people with co-morbid conditions such as diabetes and obesity and elderly persons par...
One-step process for producing prebiotic arabino-xylooligosaccharides from Brew...
Amorim, Cláudia; Silvério, Sara C.; Rodrigues, L. R.Supplementary data associated with this article can be found, in the online version, at https://doi.org/10.1016/j.foodchem.2018.07.080.; Xylooligosaccharides (XOS) are prebiotic nutraceuticals that can be sourced from lignocellulosic biomass, such as agro-residues. This study reports for the first time an optimization study of XOS production from agro-residues by direct fermentation using two Trichoderma specie...
Evaluation of disruption/permeabilization methodologies for Microcystis aerugin...
Geada, Pedro; Loureiro, Luís; Teixeira, J. A.; Vítor Vasconcelos; Vicente, A. A.; Fernandes, Bruno D.The extraction of the wide range of useful bioactive compounds produced by cyanobacteria is still a major bottleneck at industrial scale. In addition to the high costs, extraction efficiencies are also commonly low, with low cell disruption efficiencies playing a particularly significant role in intracellular compounds' release. To increase the chances of an extended use of the cyanobacteria toxin microcystin i...
Comparison and optimization of different methods for Microcystis aeruginosa's h...
Geada, Pedro; Oliveira, F.; Loureiro, Luís; Esteves, D.; Teixeira, J. A.; Vasconcelos, Vítor; Vicente, A. A.; Fernandes, Bruno D.This study has compared the harvesting efficiency of four flocculation methods, namely, induced by pH, FeCl3, AlCl3 and chitosan. No changes were observed on M. aeruginosa cells. Flocculation assays performed at pH 3 and 4 have shown the best harvesting efficiency among the pH-induced tests, reaching values above 90% after 8 h. The adjustment of zeta potential (ZP) to values comprised between 6.7 and 20.7 mV en...
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