Project/scholarship details


  • Funder

    FCT - Fundação para a Ciência e a Tecnologia, I.P.

  • Funder's country

    Portugal

  • Funding program

    5876-PPCDTI

  • Funding amount

    338,852.00 €

  • Start date

    2013-01-01

  • End date

    2015-12-31

Documents


Simulated moving bed operating conditions for fructo-oligosaccharides separation

Gonçalves, Clarisse Salomé Nobre; Teixeira, J. A.; Rodrigues, L. R.; Weireld, Guy De

Fructo-oligosaccharides (FOS) are produced with low conversion yields and purity. The fermentative broth obtained from the FOS synthesis contains, apart from FOS, small chain saccharides that do not contribute to the overall beneficial prebiotic activity of the mixture and must be removed. In this work, the separation of FOS from mono- and disaccharides by Simulated Moving Bed (SMB) chromatography was studied. ...


Perspectives on utilization of edible coatings and nano-laminate coatings for e...

López, M.; Cerqueira, Miguel Ângelo Parente Ribeiro; de Rodríguez, Diana Jasso; Vicente, A. A.

It is known that in developing countries, a large quantity of fruit and vegetable losses results at postharvest and processing stages due to poor or scarce storage technology and mishandling during harvest. The use of new and innovative technologies for reducing postharvest losses is a requirement that has not been fully covered. The use of edible coatings (mainly based on biopolymers) as a postharvest techniqu...


Toxicity induced by a metal mixture (Cd, Pb and Zn) in the yeast Pichia kudriav...

Mesquita, Vanessa A.; Silva, Cristina F.; Soares, Eduardo V.

The present work aims to contribute for the elucidation of the role of oxidative stress in the toxicity associated with the exposure of Pichia kudriavzevii to multi-metals (Cd, Pb and Zn). Cells of the non-conventional yeast P. kudriavzevii exposed for 6 h to the action of multi-metals accumulated intracellular reactive oxygen species (ROS), evaluated through the oxidation of the probe 2,7-dichlorodihydrofluore...


Production of whey protein-based aggregates under ohmic heating

Pereira, Ricardo; Rodrigues, Rui M.; Ramos, Óscar L.; Malcata, F. X.; Teixeira, J. A.; Vicente, A. A.

Formation of whey protein isolate protein aggregates under the influence of moderate electric fields upon ohmic heating (OH) has been monitored through evaluation of molecular protein unfolding, loss of its solubility, and aggregation. To shed more light on the microstructure of the protein aggregates produced by OH, samples were assayed by transmission electron microscopy (TEM). Results show that during early ...


Use of electrospinning to develop antimicrobial biodegradable multilayer system...

Cerqueira, Miguel A.; Rovira, M.; Castro-Mayorga, Jinneth Lorena; Bourbon, Ana I.; Pastrana, Lorenzo M.; Vicente, António A.; Lagaron, Jose M.

In this work, three active bio-based multilayer structures, using a polyhydroxybutyrate-co-valerate film with a valerate content of 8 % (PHBV8) as support, were developed. To this end, a zein interlayer with or without cinnamaldehyde (CNMA) was directly electrospun onto one side of the PHBV8 film and the following systems were developed: (1) without an outer layer; (2) using a PHBV8 film as outer layer; and (3)...


Valorization of agro-industrial wastes towards the production of rhamnolipids

Gudiña, Eduardo J.; Rodrigues, A.; Freitas, Victor de; Azevedo, Zélia; Teixeira, J. A.; Rodrigues, L. R.

In this work, oil mill wastewater (OMW), a residue generated during olive oil extraction, was evaluated as an inducer of rhamnolipid production. Using a medium containing as sole ingredients corn steep liquor (10%, v/v), sugarcane molasses (10%, w/v) and OMW (25%, v/v), Pseudomonas aeruginosa #112 produced 4.5 and 5.1 g of rhamnolipid per liter in flasks and reactor, respectively, with critical micelle concentr...


Beeswax organogels: Influence of gelator concentration and oil type in the gela...

Martins, Artur J.; Cerqueira, M. A.; Fasolin, L.; Cunha, Rosiane L.; Vicente, A. A.

This work focused on how different types of oil phase, MCT (medium chain triglycerides) and LCT (long chain triglycerides), exert influence on the gelation process of beeswax and thus properties of the organogel produced thereof. Organogels were produced at different temperatures and qualitative phase diagrams were constructed to identify and classify the type of structure formed at various compositions. The mi...


Approaches in biotechnological applications of natural polymers

Albuquerque, Priscilla B. S.; Coelho, Luana C. B. B.; Teixeira, J. A.; Carneiro-da-Cunha, M. G.

Natural polymers, such as gums and mucilage, are biocompatible, cheap, easily available and non-toxic materials of native origin. These polymers are increasingly preferred over synthetic materials for industrial applications due to their intrinsic properties, as well as they are considered alternative sources of raw materials since they present characteristics of sustainability, biodegradability and biosafety. ...


Discrimination of bacteriophage infected cells using locked nucleic acid fluore...

Boas, Diana Patrícia Andrade Vilas; Almeida, Carina; Sillankorva, Sanna; Nicolau, Ana; Azeredo, Joana; Azevedo, Nuno F.

Bacteriophage-host interaction studies in biofilm structures are still challenging due to the technical limitations of traditional methods. The aim of this study was to provide a direct fluorescence in situ hybridization (FISH) method based on locked nucleic acid (LNA) probes, which targets the phage replication phase, allowing the study of population dynamics during infection. Bacteriophages specific for two b...


Polydopamine-mediated immobilization of alginate lyase to prevent P. aeruginosa...

Alves, D.; Sileika, Tadas; Messersmith, Phillip; Pereira, Maria Olívia

Given alginate’s contribution to Pseudomonas aeruginosa virulence, it has long been considered a promising target for interventional therapies, which have been performed by using the enzyme alginate lyase. In this work, instead of treating pre-established mucoid biofi lms, alginate lyase is immobilized onto a surface as a preventive measure against P. aeruginosa adhesion. A polydopamine dip-coating strategy is e...

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