15 documents found, page 1 of 2
Selection and characterization of peptides mimetic to Campylobacter fetus subsp...
Ribeiro,Eduardo Lara; Fujimura,Patrícia Tiemi; Ueira-Vieira,Carlos; Goulart,Luiz Ricardo; Alves,Telma Maria; Lage,Andrey Pereira; Heinemann,Marcos BryanABSTRACT: Bovine genital campylobacteriosis (BGC) is a venereal and subclinical disease that affects the fertility of cattle herds, and it is caused by Campylobacter fetus subsp. venerealis . This study selected peptides mimetic to the BGC-causing agent from a phage library. Phage display is a technique that applies bacteriophage libraries that reveal peptides fused to the viral capsid in biological selections ...
Invasive mechanical ventilation and biomarkers as predictors of bronchopulmonar...
Nascimento,Camila Piqui; Maia,Larissa Prado; Alves,Patrícia Terra; Paula,Aline Teodoro de; Cunha Junior,Jair Pereira; Abdallah,Vânia Olivetti SteffenAbstract Objectives To evaluate the impact of invasive mechanical ventilation associated with two serum inflammatory cytokines and clinical indicators, on the second day of life, as predictors of bronchopulmonary dysplasia in very low birth weight preterm infants. It was hypothesized that the use of invasive mechanical ventilation in the first hours of life is associated with biomarkers that may predict the cha...
Innovative approaches to improve serodiagnosis of Strongyloides stercoralis inf...
Levenhagen,Marcelo Arantes; Fujimura,Patrícia Tiemi; Santos,Fabiana de Almeida Araújo; Vecchi,Lara; Alves-Balvedi,Renata PereiraABSTRACT Strongyloidiasis is a helminthiasis of neglected condition that has no gold standard parasitological diagnosis due to the intermittent release of larvae in feces. This study aimed to use an scFv (single chain variable fragment) obtained by Phage Display, previously validated to detect immune complexes in serum samples from individuals infected with Strongyloides stercoralis by enzyme-linked immunosorbe...
Epigenetic modifications and their relation to caste and sex determination and ...
Cardoso-Júnior,Carlos A.M.; Fujimura,Patrícia Tieme; Santos-Júnior,Célio Dias; Borges,Naiara Araújo; Ueira-Vieira,Carlos; Hartfelder,KlausAbstract Stingless bees of the genus Melipona, have long been considered an enigmatic case among social insects for their mode of caste determination, where in addition to larval food type and quantity, the genotype also has a saying, as proposed over 50 years ago by Warwick E. Kerr. Several attempts have since tried to test his Mendelian two-loci/two-alleles segregation hypothesis, but only recently a single g...
Recent updates and perspectives on approaches for the development of vaccines a...
Duarte,Mariana Costa; Lage,Daniela Pagliara; Martins,Vívian Tamietti; Chávez-Fumagalli,Miguel Angel; Roatt,Bruno Mendes; Menezes-Souza,DanielAbstract: Visceral leishmaniasis (VL) is one of the most important tropical diseases worldwide. Although chemotherapy has been widely used to treat this disease, problems related to the development of parasite resistance and side effects associated with the compounds used have been noted. Hence, alternative approaches for VL control are desirable. Some methods, such as vector control and culling of infected dog...
Theranostic applications of phage display to control leishmaniasis: selection o...
Coelho,Eduardo Antonio Ferraz; Chávez-Fumagalli,Miguel Angel; Costa,Lourena Emanuele; Tavares,Carlos Alberto Pereira; Soto,Manuel; Goulart,Luiz RicardoAbstractPhage display is a high-throughput subtractive proteomic technology used for the generation and screening of large peptide and antibody libraries. It is based on the selection of phage-fused surface-exposed peptides that recognize specific ligands and demonstrate desired functionality for diagnostic and therapeutic purposes. Phage display has provided unmatched tools for controlling viral, bacterial, fu...
Risk-benefit assessment of Bacillus Calmette-Guérin vaccination, anti-phenolic ...
Araujo,Sergio; Rezende,Marina Monteiro Figueiredo; Sousa,Diogo Carrijo Rodrigues de; Rosa,Maraísa Resende; Santos,Danielle Cristina dosAbstract: INTRODUCTION: Despite multidrug therapy, leprosy remains a public health issue. The intradermal Bacillus Calmette-Guérin (BCG) vaccine, Mitsuda test (lepromin skin test), and anti-phenolic glycolipid I (PGL-I) serology are widely used in leprosy studies and have shown great epidemiological value. METHODS: This longitudinal study evaluated the relative risks and benefits of these three tools by compari...
In silico analysis for identification of tick phagotopes selected by phage-disp...
Prudencio,Carlos Roberto; Nascimento,Rafael; Marchiori Filho,Moacir; Marra,Andrea de Oliveira Marques; Souza,Guilherme Rocha Lino dePhage display techniques have been widely employed to map epitope structures which have served as the basis for developing molecular vaccines. We have applied this technique to map specific epitopes of Rhipicephalus (Boophilus) microplus. In the present study, we have identified the potential immunogens using a process in which the selected phage clones were analyzed through bioinformatics, prior to final field...
Association of the estrogen receptor gene Pvu II restriction polymorphism with ...
Santana,Bárbara Amélia Aparecida; Biase,Fernando H.; Antunes,Robson Carlos; Borges,Mauricio; Franco,Mauricio Machaim; Goulart,Luiz RicardoEstrogen has an important function in swine reproduction and growth. A Pvu II restriction enzyme polymorphism has been proven to be an important genetic variation in the estrogen receptor gene (ESR) and may be considered as a candidate gene for use in pig production but there is no data regarding the prevalence of this polymorphism in the Brazilian pig population. We used DNA samples from the following three pu...
Association of a PIT1 gene polymorphism with growth hormone mRNA levels in pig ...
Franco,Maurício Machaim; Antunes,Robson Carlos; Oliveira,Katiana Mello de; Pereira,Cícero Donizete; Biase,Fernando HenriqueFourty-six non-castrated, halothane-free, male Landrace pigs were genotyped by PCR-RFLP for the Rsa I polymorphism in the PIT1 gene and classified into AA and AB genotypes. Total RNA was extracted from the pituitaries and the relative quantities of growth hormone (GH) mRNA were determined by semi-quantitative RT-PCR. Pigs with the AB genotype had higher levels of GH mRNA than those with the AA genotype (p = 0.0...