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Suitable reference genes for RT-qPCR analysis in Dichelops melacanthus (Hemipte...
Pinheiro, Daniele Heloísa; Moreira, Raquel Oliveira [UNESP]; Leite, Natália Alves; Redoan, Ana Carolina; Xavier, André da SilvaMade available in DSpace on 2020-12-12T01:29:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-07-01; The relative quantification of gene expression is mainly realized through reverse transcription-quantitative PCR (RT-qPCR). However, the accuracy of this technique is deeply influenced by the expression stability of the reference genes used for data normalization. Therefore, the selection of suitable re...
The complete genome sequence of Chromobacterium violaceum reveals remarkable an...
Vasconcelos, Ana Tereza; De-Almeida, Darcy Fontoura; Hungría, Mariangela; Guimarães, Cláudia Teixeira; Antônio, Regina VasconcellosChromobacterium violaceum is one of millions of species of free-living microorganisms that populate the soil and water in the extant areas of tropical biodiversity around the world. Its complete genome sequence reveals (i) extensive alternative pathways for energy generation, (ii) ≈500 ORFs for transport-related proteins, (iii) complex and extensive systems for stress adaptation and motility, and (iv) wide-spre...
Molecular caracterization of a corn core collection–landraces subgroup
Miranda, Glauco Vieira; Coimbra, Ronaldo Rodrigues; Carneiro, Newton Portilho; Guimarães, Cláudia Teixeira; Silva, Derly José Henriques daA utilização eficiente de recursos genéticos, armazenados em coleções de germoplasma, pode ser maximizada se informações morfo-agronômicas e moleculares sobre os acessos forem disponibilizadas. Para isso, é necessária a existência de uma coleção racionalizada, refinada e estruturada, chamada de coleção núcleo. Assim, objetivou-se com este trabalho caracterizar molecularmente 80 acessos landraces da coleção núcl...
Agrobacterium-mediated genetic transformation of a tropical elite maize line
Souza, Rafaeli Aparecida Vieira de; Alves, Meire de Cássia; Carneiro, Newton Portilho; Barros, Beatriz de Almeida; Borém, AluízioO artigo não contém resumo em português; The efficiency of maize transformation mediated by Agrobacterium tumefaciens is influenced by various factors. The aim of this study was to test the effect of different concentrations of N6 salts (50% - half strength, and 100% - full strength) in the infection and cocultivation media on genetic transformation efficiency of the L3 tropical elite maize line. Immature embry...
Molecular caracterization of a corn core collection–landraces subgroup
Coimbra, Ronaldo Rodrigues; Miranda, Glauco Vieira; Carneiro, Newton Portilho; Guimarães, Cláudia Teixeira; Henriques da Silva, Derly JoséThe efficient use of genetic resources- stored in germplasm collections can be maximized if morphoagronomic and molecular information on the accessions is made available. To achieve this, a collection that is well-structured, well-curated and easily accessible (the core collection) is required. Consequently, the objective of the current study was to characterize 80 landrace accessions from the maize core collec...
Evidence for Reductive Genome Evolution and Lateral Acquisition of Virulence Fu...
Ruiz, Jerônimo Conceição; Ferreira, Vívian D'Afonseca da Silva; Silva, Artur Luiz da Costa da; Ali, Amjad; Pinto, Anne Cybellep. 1-15; Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-02-27T13:03:23Z No. of bitstreams: 1 journal.pone.0018551.pdf: 410957 bytes, checksum: 9150a5f0fbe637d4f1197a80e401c8d2 (MD5); Made available in DSpace on 2012-02-27T13:03:23Z (GMT). No. of bitstreams: 1 journal.pone.0018551.pdf: 410957 bytes, checksum: 9150a5f0fbe637d4f1197a80e401c8d2 (MD5) Previous issue date: 2011; Background: Coryneb...