5 documents found, page 1 of 1
Spillover events of rabbit haemorrhagic disease virus 2 (recombinant GI.4P-GI.2...
Abade dos Santos, F.A.; Pinto, Andreia; Burgoyne, Thomas; Dalton, Kevin P.; Carvalho, Carina L.; Ramilo, David; Carneiro, Carla; Carvalho, TâniaRabbit haemorrhagic disease (RHD) is a major threat to domestic and wild European rabbits. Presently, in Europe, the disease is caused mainly by Rabbit haemorrhagic disease virus 2 (RHDV2/b or Lagovirus europaeus GI.2), the origin of which is still unclear, as no RHDV2 reservoir hosts were identified. After the RHDV2 emergence in 2010, viral RNA was detected in a few rodent species. Furthermore, RHDV2 was found...
Simple method for establishing primary Leporidae skin fibroblast cultures
Abade dos Santos, F.A.; Carvalho, C. L.; Almeida, Isabel; FAGULHA, TERESA; Ramos, Fernanda; Barros, Sílvia C.; Henriques, Margarida; Luís, TiagoCommercial hare and rabbit immortalized cell lines are extremely limited regarding the many species within the lagomorpha order. To overcome this limitation, researchers and technicians must establish primary cell cultures derived from biopsies or embryos. Among all cell types, fibroblasts are plastic and resilient cells, highly convenient for clinical and fundamental research but also for diagnosis, particular...
Harmless or threatening? Interpreting the results of molecular diagnosis in the...
Abade dos Santos, F.A.; Portela, Sara J.; Nogueira, Teresa; Carvalho, Carina L.; Sousa, Rita de; Duarte, M. D.Molecular methods, established in the 1980s, expanded and delivered tools for the detection of vestigial quantities of nucleic acids in biological samples. Nucleotide sequencing of these molecules reveals the identity of the organism it belongs to. However, the implications of such detection are often misinterpreted as pathogenic, even in the absence of corroborating clinical evidence. This is particularly sign...
First description of a herpesvirus infection in genus Lepus
Abade dos Santos, F.A.; M. Monteiro, A. Pinto; Carvalho, C. L.; Peleteiro, Maria C.ABSTRACT - During the necropsies of Iberian hares obtained in 2018/2019, along with signs of the nodu- lar form of myxomatosis, other unexpected external lesions were also observed. Histopa- thology revealed nuclear inclusion bodies in stromal cells suggesting the additional presence of a nuclear replicating virus. Transmission electron microscopy further demon- strated the presence of herpesvirus particles in ...
Nutritional value of meat lipid fraction from red-legged partridge (Alectoris r...
Antunes, IC; Coimbra, MCP; Ribeiro, AP; Ferreira, JD; Abade dos Santos, F.A.; Alves, SP; Bessa, RJB; Quaresma, MAGABSTRACT - The red-legged partridge (Alectoris rufa) is a feathered game species of great socioeconomic importance in its native range and also in the UK. The aim of this study was to present a detailed comparison of meat's lipid fraction obtained from wild and farm-raised specimens and simultaneously compare the breast and leg meat portions. Meat from wild specimens had a significant (P < 0.05) lower proportio...