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A simple PCR-based procedure for plaque diagnosis
Leal, Nilma Cintra; Abath, Frederico Guilherme Coutinho; Alves, Luis Carlos; Almeida, Alzira Maria Paiva deSupernatant of boiled spleen saline-suspensions of Yersinia pestis experimentally infected animals were used as template for PCR amplification without DNA extraction. PCR sensitivity was enhanced by a second round of amplification (Nested). No amplification was observed from non-infected animals.; Triturados de baços de animais infectados experimentalmente com Y. pestis, suspensos em salina foram fervidos e os ...
Comparative studies of Yersinia pestis outer membrane isolation techniques and ...
Abath, Frederico Guilherme Coutinho; Ferreira, Luís Carlos de SousaIn the present study three techniques for obtaining outer membrane enriched fractions from Yersinia pestis were evaluated. The techniques analysed were: differential solubilization of the cytoplasmic membrane with Sarkosyl or Triton X-100, and centrifugation in sucrose density gradients. The sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of outer membrane isolated by the different methods ...