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Recovering polyploid papaya in vitro regenerants as screened by flow cytometry
Clarindo, Wellington Ronildo; Carvalho, Carlos Roberto de; Araújo, Fernanda Santos; Abreu, Isabella Santiago de; Otoni, Wagner CamposSeveral protocols have been proposed for in vitro propagation of papaya, either based on somatic embryogenesis or shoot organogenesis. It is well-known that tissue culture-based approaches are frequently associated with somaclonal variation. Whether on the one hand this phenomenon can preclude further stages of in vitro culture, on the other hand it can generate useful genetic variability for crop improvement. ...
Chromosomal DNA content of sweet pepper determined by association of cytogeneti...
Abreu, Isabella Santiago de; Carvalho, Carlos Roberto; Clarindo, Wellington RonildoThe nuclear DNA content of sweet pepper (Capsicum annuum L. var. annuum, 2n = 24) has been measured by flow and image cytometries but the DNA content of each chromosome of this species has not yet been regarded. DNA content of individual chromosomes has been quantified by the flow karyotyping technique, which requires a great quantity of intact metaphasic chromosomes and methods that allow the characterization ...
Monitoramento da embriogênese somática de Carica papaya L. por técnicas citogen...
Abreu, Isabella Santiago deCoordenação de Aperfeiçoamento de Pessoal de Nível Superior; Somatic embryogenesis is an alternative technique of clonal micropropagation in Carica papaya, taking into account the problems generated by the conventional seed propagation and the absence of an effective method for early sex determination of this trioecious species. Considering the interest in large-scale seedlings production of papaya, liquid syst...