172 documents found, page 1 of 18
Recombinant bovine serum albumin domain II as bioreceptor for ochratoxin A capture
Aguiar, Tatiana Quinta; Leal, Tânia; Rodrigues, Diana G.; Abrunhosa, Luís; Oliveira, Carla; Domingues, LucíliaEstablished chromatographic techniques for mycotoxin control in foodstuffs require prior sample enrichment and clean-up, typically achieved using immunoaffinity columns (IACs). Bovine serum albumin (BSA) has recently emerged as a cost-effective alternative to antibodies used in IACs. This study aimed at exploring the BSA domain II (BDII), which houses the primary binding site for ochratoxin A (OTA), as a biorec...
Novel recombinant Human Serum Albumin-SPE systems for ochratoxin A determinatio...
Rodrigues, Diana; Aguiar, Tatiana Quinta; Domingues, Lucília; Abrunhosa, LuísOchratoxin A (OTA) is a mycotoxin that raises food safety concerns in several products, requiring analytical control. Aiming to develop an alternative to immunoaffinity columns (IACs) for OTA extraction and clean-up, recombinant human serum albumin (rHSA) was explored as an affinity bioreceptor for solid-phase extraction (SPE) applications. rHSA was obtained from Pichia pastoris fused with a C-terminal His6 tag...
Innovations in food testing tech: ensuring safety from chemical and microbial c...
Rodrigues, Rui Miguel Martins; Abrunhosa, Luís; Homem, VeraFood safety is a key priority in the food industry due to the health and economic risks from contamination and adulteration. Each year, millions of food samples are tested worldwide to detect contaminants, verify labeling and ensure compliance with safety regulations. This article explores the latest advancements in food testing from cutting-edge chemical analysis to AI-driven data analytics highlighting how th...
Microplate fluorescence quenching for high throughput screening of affinity con...
Adalsteinsson, Heidar Már; Pinto, Lavínia Anjos Salazar Quartin; Aguiar, Tatiana Quinta; Teixeira, J. A.; Abrunhosa, LuísMeasurements of changes in fluorescence signal is one of the most commonly applied methods for studying protein-ligand affinities. These measurements are generally carried out using cuvettes in spectrofluorometers, which can only measure one sample at a time. This makes screening procedures for multiple ligands and proteins extremely laborious, as each protein must be measured with multiple ligand concentration...
Recombinant bovine serum albumin domain II as bioreceptor for ochratoxin A capture
Aguiar, Tatiana Q.; Leal, Tânia; Rodrigues, Diana G.; Abrunhosa, Luís; Oliveira, Carla; Domingues, LucíliaEstablished chromatographic techniques for mycotoxin control in foodstuffs require prior sample enrichment and clean-up, typically achieved using immunoaffinity columns (IACs). Bovine serum albumin (BSA) has recently emerged as a cost-effective alternative to antibodies used in IACs. This study aimed at exploring the BSA domain II (BDII), which houses the primary binding site for ochratoxin A (OTA), as a biorec...
Valorization of food by-products via solid submerged fermentation for sustainab...
Sousa, Vítor Emanuel Silva; Coelho, Eduardo João Louro; Vicente, A. A.; Abrunhosa, LuísBackground: The growing demand for sustainable protein sources has intensified interest in alternative production systems with reduced environmental impact. Mycoprotein is a promising option in this context. This study explored solid submerged fermentation (SSmF) as a bioprocess for producing mycoprotein from Pleurotus spp. using food by-products. Brewers spent grain (BSG), banana peel (BP), peanut peel (PL), a...
Potential prebiotic effect of Caatinga bee honeys from the Pajeú hinterland (Pe...
Pinto-Neto, Walter de Paula; Loureiro, Luís Carlos Sá; Gonçalves, Raquel Filipa Silva; Marques, Márcia Cristina Teixeira; Rodrigues, Rui Miguel MartinsThe singular biodiversity of the Brazilian Caatinga inspires innovative solutions in food science. In this study, we evaluated the prebiotic potential of honeys produced by Apis mellifera in the Pajeú hinterland, Pernambuco, Brazil (Caatinga Biome), with different floral origins: Mastic (Aroeira), Mesquite (Algaroba), and mixed flowers. These were used to formulate synbiotic and alcoholic beverages fermented by...
Occurrence of aflatoxins and ochratoxin A in dried figs from the Algarve region
Mendes, Cíntia; Abrunhosa, LuísFigs are highly susceptible to fungal contamination and, consequently, dried figs may contain mycotoxins such as aflatoxins (AFLs) and ochratoxin A (OTA). These compounds are mainly produced by mycotoxigenic species of the genera Aspergillus and Penicillium. In the European Union, the legal limit for AFLs in dried figs is 10 µg/kg, while the limit for OTA is 8 µg/kg [1]. Therefore, regular analytical control of...
Co-encapsulation of vitamin D3 and curcumin in plant protein-based nanoemulsion...
Marques, Márcia; Gonçalves, Raquel Filipa Silva; Madalena, Daniel Alexandre Silva; Abrunhosa, Luís; Vicente, A. A.; Pinheiro, A. C.This study aimed to develop plant-based nanoemulsions (NE) for co-encapsulation of vitamin D3 and curcumin, comparing the emulsifying properties of pea and potato proteins. Different oil and protein concentrations were tested, and formulations with the smallest particle size were characterized and submitted to harmonized static in vitro digestion. Formulations prepared with the highest protein concentration (10...
Toward cost-effective production of recombinant human serum albumin for use in ...
Rodrigues, Diana Isabel Gomes; Adalsteinsson, Heidar Már; Pinto, Lavínia; Aguiar, Tatiana Quinta; Oliveira, Carla; Abrunhosa, Luís; Domingues, LucíliaHuman serum albumin (HSA) is a monomeric multidomain macromolecule with wide clinical and biotechnological applications. Thus, there is a high demand for HSA, which is increasing due to new emerging applications. Among these, the extraordinary ligand binding capacity of HSA has been recently explored to capture mycotoxins from food and feed matrices. However, HSA is currently produced commercially primarily fro...