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Meta-analysis of antimicrobial activity of Allium, Ocimum, and Thymus spp. conf...

Bonilla-Luque, Olga María; Silva, Beatriz Nunes; Ezzaky, Youssef; Possas, Arícia; Achemchem, Fouad; Cadavez, Vasco; Gonzales-Barron, Ursula

Biopreservation strategies such as the use of Mediterranean plant extracts to ensure food safety are promising to deal with the emergence of antimicrobial resistances and the overreliance on food chemical additives. In the last few decades, antimicrobial susceptibility testing (AST) for evaluating the in vitro antibacterial potential of plant extracts against the most relevant foodborne pathogens has been widel...

Date: 2024   |   Origin: Biblioteca Digital do IPB

Shotgun metagenomic investigation of foodborne pathogens and antimicrobial resi...

Indio, Valentina; Olivieri, Chiara; Lucchi, Alex; Savini, Federica; Gonzales-Barron, Ursula; Skandamis, Panagiotis; Achemchem, Fouad; Manfreda, Gerardo

In this pilot study, we compared the metagenomic profiles of different types of artisanal fermented meat products collected in Italy, Greece, Portugal, and Morocco to investigate their taxonomic profile, also in relation to the presence of foodborne pathogens and antimicrobial resistance genes. In addition, technical replicates of the same biological sample were tested to estimate the reproducibility of shotgun...

Date: 2024   |   Origin: Biblioteca Digital do IPB

Meta-analysis of antimicrobial activity of Allium, Ocimum, and Thymus spp. conf...

Bonilla-Luque, Olga María; Silva, Beatriz Nunes; Ezzaky, Youssef; Possas, Arícia; Achemchem, Fouad; Cadavez, Vasco; Gonzales-Barron, Úrsula

Biopreservation strategies such as the use of Mediterranean plant extracts to ensure food safety are promising to deal with the emergence of antimicrobial resistances and the overreliance of food chemical additives. In the last few decades, antimicrobial susceptibility testing (AST) for evaluating the in vitro antibacterial potential of plant extracts against the most relevant foodborne pathogens has been widel...


In vitro antimicrobial activity of extracts and essential oils of Cinnamomum, S...

Ezzaky, Youssef; Elmoslih, Abdelkhaleq; Silva, Beatriz Nunes; Bonilla‐Luque, Olga M.; Possas, Arícia; Valero, Antonio; Cadavez, Vasco

Genetic analysis of the honey bee spermathecal content can be particularly useful to provide an estimate of the genetic diversity and purity of the surrounding populations. Here we compared the concentration and quality of DNA extracted from queen spermatheca using four commercial kits to determine the best method to obtain DNA suitable for single nucleotide polymorphism genotyping by next-generation sequencing...

Date: 2023   |   Origin: Biblioteca Digital do IPB

In vitro antimicrobial activity of extracts and essential oils of Cinnamomum, S...

Ezzaky, Youssef; Elmoslih, Abdelkhaleq; Silva, Beatriz Nunes; Bonilla‐Luque, Olga María; Possas, Arícia; Valero, Antonio; Cadavez, Vasco

Essential oils (EOs) are a class of natural products that exhibit potent antimicrobial properties against a broad spectrum of bacteria. Inhibition diameters (IDs) and minimum inhibitory concentrations (MICs) are the typical measures of antimicrobial activity for extracts and EOs obtained from Cinnamomum, Salvia, and Mentha species. This study used a meta-analytical regression analysis to investigate the correla...

Date: 2023   |   Origin: Biblioteca Digital do IPB

Meta-analysis of in vitro antimicrobial capacity of extracts and essential oils...

Silva, Beatriz Nunes; Bonilla-Luque, Olga María; Possas, Arícia; Ezzaky, Youssef; Elmoslih, Abdelkhaleq; Teixeira, José António; Achemchem, Fouad

Diffusion methods, including agar disk-diffusion and agar well-diffusion, as well as dilution methods such as broth and agar dilution, are frequently employed to evaluate the antimicrobial capacity of extracts and essential oils (EOs) derived from Origanum L., Syzygium aromaticum, and Citrus L. The results are reported as inhibition diameters (IDs) and minimum inhibitory concentrations (MICs), respectively. In ...

Date: 2023   |   Origin: Biblioteca Digital do IPB

In vitro antimicrobial activity of extracts and essential oils of Cinnamomum, S...

Ezzaky, Youssef; Elmoslih, Abdelkhaleq; Silva, Beatriz Nunes; Bonilla-Luque, Olga María; Possas, Arícia; Valero, Antonio; Cadavez, Vasco

Abstract Essential oils (EOs) are a class of natural products that exhibit potent antimicrobial properties against a broad spectrum of bacteria. Inhibition diameters (IDs) and minimum inhibitory concentrations (MICs) are the typical measures of antimicrobial activity for extracts and EOs obtained from Cinnamomum, Salvia, and Mentha species. This study used a meta-analytical regression analysis to investigate th...


Meta-analysis of in vitro antimicrobial capacity of extracts and essential oils...

Silva, Beatriz Nunes; Bonilla-Luque, Olga María; Possas, Arícia; Ezzaky, Youssef; Elmoslih, Abdelkhaleq; Teixeira, J. A.; Achemchem, Fouad

Diffusion methods, including agar disk-diffusion and agar well-diffusion, as well as dilution methods such as broth and agar dilution, are frequently employed to evaluate the antimicrobial capacity of extracts and essential oils (EOs) derived from Origanum L., Syzygium aromaticum, and Citrus L. The results are reported as inhibition diameters (IDs) and minimum inhibitory concentrations (MICs), respectively. In ...


Resistome and virulome diversity of foodborne pathogens isolated from artisanal...

Pasquali, Frédérique; Gambi, Lucia; Cesare, Alessandra; Crippa, Cecilia; Cadavez, Vasco; Gonzales-Barron, Ursula; Valero, Antonio; Achemchem, Fouad

The aim of the present study was to investigate the resistome and virulome diversity of 43 isolates of Listeria monocytogenes, Salmonella enterica and S. aureus collected from artisanal fermented meat and dairy products and their production environments in Portugal, Spain, Italy and Morocco. After DNA extraction, genomes were sequenced, and de novo assembled. Genetic relationships among genomes were investigate...

Date: 2022   |   Origin: Biblioteca Digital do IPB

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