4 documents found, page 1 of 1
RIPK3 dampens mitochondrial bioenergetics and lipid droplet dynamics in metabol...
Afonso, Marta B.; Islam, Tawhidul; Magusto, Julie; Amorim, Ricardo; Lenoir, Véronique; Simões, Rui F.; Teixeira, José; Silva, Liana C.Background and Aims: Receptor‐interacting protein kinase 3 (RIPK3) mediates NAFLD progression, but its metabolic function is unclear. Here, we aimed to investigate the role of RIPK3 in modulating mitochondria function, coupled with lipid droplet (LD) architecture in NAFLD. Approach and Results: Functional studies evaluating mitochondria and LD biology were performed in wild‐type (WT) and Ripk3−/− mice fed a cho...
Disclosing the antitumour potential of the marine bromoditerpene sphaerococceno...
Alves, Celso; Silva, Joana; Afonso, Marta B.; Guedes, Romina A.; Guedes, Rita C.; Alvariño, Rebeca; Pinteus, Susete; Gaspar, Helena; Goettert, Marcia I.Nature has revealed to be a key source of innovative anticancer drugs. This study evaluated the antitumour potential of the marine bromoditerpene sphaerococcenol A on different cancer cellular models. Dose-response analyses (0.1–100 μM; 24 h) were accomplished in eight different tumour cell lines (A549, CACO-2, HCT-15, MCF-7, NCI-H226, PC-3, SH-SY5Y, SK-MEL-28). Deeper studies were conducted on MFC-7 cells, nam...
Antitumour Potential of Gigartina pistillata Carrageenans against Colorectal Ca...
Cotas, João; Marques, Vanda; Afonso, Marta B.; Rodrigues, Cecília M. P.; Pereira, LeonelGigartina pistillata is a red seaweed common in Figueira da Foz, Portugal. Here, the antitumour potential of G. pistillata carrageenan, with a known variable of the life cycle, the female gametophyte (FG) and tetrasporophyte (T) was evaluated against colorectal cancer stem cell (CSC) -enriched tumourspheres. FTIR-ATR analysis of G. pistillata carrageenan extracts indicated differences between life cycle phases,...
miRNA-21 ablation protects against liver injury and necroptosis in cholestasis
Afonso, Marta B.; Rodrigues, Pedro M.; Simão, André L.; Gaspar, Maria M.; Carvalho, Tânia; Borralho, Paula; Bañales, Jesús M.; Castro, Rui E.Inhibition of microRNA-21 (miR-21) prevents necroptosis in the mouse pancreas. Necroptosis contributes to hepatic necro-inflammation in the common bile duct ligation (BDL) murine model. We aimed to evaluate the role of miR-21 in mediating deleterious processes associated with cholestasis. Mechanistic studies established a functional link between miR-21 and necroptosis through cyclin-dependent kinase 2-associate...