10 documents found, page 1 of 1
Heterocellular Contacts with Mouse Brain Endothelial Cells Via Laminin and α6β1...
Rosa, Alexandra I.; Grade, Sofia; Santos, Sofia D.; Bernardino, Liliana; Chen, Thomas C.; Relvas, João; Hofman, Florence M.; Agasse, FabienneNeurogenesis in the subventricular zone (SVZ) is regulated by diffusible factors and cell-cell contacts. In vivo, SVZ stem cells are associated with the abluminal surface of blood vessels and such interactions are thought to regulate their neurogenic capacity. SVZ neural stem cells (NSCs) have been described to contact endothelial-derived laminin via α6β1 integrin. To elucidate whether heterocellular contacts w...
Growth hormone pathways signaling for cell proliferation and survival in hippoc...
Devesa, Pablo; Agasse, Fabienne; Xapelli, Sara Alves; Almengló, Cristina; Devesa, Jesús; Malva, João O.; Arce, Víctor MBackground: Accumulating evidence suggests that growth hormone (GH) may play a major role in the regulation of postnatal neurogenesis, thus supporting the possibility that it may be also involved in promoting brain repair after brain injury. In order to gain further insight on this possibility, in this study we have investigated the pathways signaling the effect of GH treatment on the proliferation and survival...
Modulation of subventricular zone oligodendrogenesis: a role for hemopressin?
Xapelli, Sara Alves; Agasse, Fabienne; Grade, Sofia; Bernardino, Liliana; Ribeiro, Filipa F.; Schitine, Clarissa S.; Heimann, Andrea S.; Ferro, Emer S.Neural stem cells (NSCs) from the subventricular zone (SVZ) have been indicated as a source of new oligodendrocytes to use in regenerative medicine for myelin pathologies. Indeed, NSCs are multipotent cells that can self-renew and differentiate into all neural cell types of the central nervous system. In normal conditions, SVZ cells are poorly oligodendrogenic, nevertheless their oligodendrogenic potential is b...
Methamphetamine decreases dentate gyrus stem cell self-renewal and shifts the d...
Baptista, Sofia; Lasgi, Charlène; Benstaali, Caroline; Milhazes, Nuno; Borges, Fernanda; Fontes-Ribeiro, Carlos; Agasse, Fabienne; Silva, Ana PaulaMethamphetamine (METH) is a highly addictive psychostimulant drug of abuse that negatively interferes with neurogenesis. In fact, we have previously shown that METH triggers stem/progenitor cell death and decreases neuronal differentiation in the dentate gyrus (DG). Still, little is known regarding its effect on DG stem cell properties. Herein, we investigate the impact of METH on mice DG stem/progenitor cell s...
Activation of type 1 cannabinoid receptor (CB1R) promotes neurogenesis in murin...
Xapelli, Sara Alves; Agasse, Fabienne; Sardà-Arroyo, Laura; Bernardino, Liliana; Santos, Tiago; Ribeiro, Filipa F.; Valero, Jorge; Bragança, JoséThe endocannabinoid system has been implicated in the modulation of adult neurogenesis. Here, we describe the effect of type 1 cannabinoid receptor (CB1R) activation on self-renewal, proliferation and neuronal differentiation in mouse neonatal subventricular zone (SVZ) stem/progenitor cell cultures. Expression of CB1R was detected in SVZ-derived immature cells (Nestin-positive), neurons and astrocytes. Stimulat...
Activation of Type 1 Cannabinoid Receptor (CB1R) promotes neurogenesis in murin...
Xapelli, Sara; Agasse, Fabienne; Sarda-Arroyo, Laura; Bernardino, Liliana; Santos, Tiago; Ribeiro, Filipa F.; Valero, Jorge; Braganca, JoséThe endocannabinoid system has been implicated in the modulation of adult neurogenesis. Here, we describe the effect of type 1 cannabinoid receptor (CB1R) activation on self-renewal, proliferation and neuronal differentiation in mouse neonatal subventricular zone (SVZ) stem/progenitor cell cultures. Expression of CB1R was detected in SVZ-derived immature cells (Nestin-positive), neurons and astrocytes. Stimulat...
Histamine modulates microglia function
Ferreira, Raquel; Santos, Tiago; Gonçalves, Joana; Baltazar, Graça; Ferreira, Lino; Agasse, Fabienne; Bernardino, LilianaBackground: Histamine is commonly acknowledged as an inflammatory mediator in peripheral tissues, leaving its role in brain immune responses scarcely studied. Therefore, our aim was to uncover the cellular and molecular mechanisms elicited by this molecule and its receptors in microglia-induced inflammation by evaluating cell migration and inflammatory mediator release. Methods: Firstly, we detected the express...
Blockade of adenosine A2A receptors prevents interleukin-1β-induced exacerbatio...
Simões, Ana Patrícia; Duarte, João A; Agasse, Fabienne; Canas, Paula M.; Tomé, Ângelo R.; Agostinho, Paula; Cunha, Rodrigo A.Background and purpose: Blockade of adenosine A2A receptors (A2AR) affords robust neuroprotection in a number of brain conditions, although the mechanisms are still unknown. A likely candidate mechanism for this neuroprotection is the control of neuroinflammation, which contributes to the amplification of neurodegeneration, mainly through the abnormal release of pro-inflammatory cytokines such as interleukin(IL...
Histamine stimulates neurogenesis in the rodent subventricular zone
Bernardino, Liliana; Eiriz, Maria Francisca; Santos, Tiago; Xapelli, Sara; Grade, Sofia; Rosa, Alexandra Isabel; Cortes, Luisa; Ferreira, RaquelNeural stem/progenitor cells present in the subventricular zone (SVZ) are a potential source of repairing cells after injury. Therefore, the identification of novel players that modulate neural stem cells differentiation can have a huge impact in stem cell-based therapies. Herein, we describe a unique role of histamine in inducing functional neuronal differentiation from cultured mouse SVZ stem/progenitor cells...
Tumor necrosis factor-alpha modulates survival, proliferation, and neuronal dif...
Bernardino, Liliana; Agasse, Fabienne; Silva, Bruno; Ferreira, Raquel; Grade, Sofia; Malva, João O.Tumor necrosis factor (TNF)- has been reported to modulate brain injury, but remarkably, little is known about its effects on neurogenesis. We report that TNF- strongly influences survival, proliferation, and neuronal differentiation in cultured subventricular zone (SVZ) neural stem/progenitor cells derived from the neonatal P1-3 C57BL/6 mice. By using single-cell calcium imaging, we developed a method, based o...