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Use of elisa employing homologous and heterologous antigens for the detection o...
Ribeiro, Flávia Coelho; Schubach, Armando de O.; Mouta-Confort, Eliame; Pacheco, Tânia M.V.; Madeira, Maria de Fátima; Abboud, Luiz Cláudio de SouzaIndirect immunofluorescence is the method recommended for the diagnosis of visceral leishmanisis in dogs, however, the accuracy of this technique is low and its use on a large scale is limited. Since ELISA does not present these limitations, this technique might be an option for the detection of IgG or specific IgG1 and IgG2 subclasses. Canine ehrlichiosis is an important differential diagnosis of American Visc...
Immunoperoxidase technique using an anti-Leishmania (L.) chagasi hyperimmune se...
Quintella, Leonardo P.; Cuzzi, Tullia; Madeira, Maria de F.; Okamoto, Thais; Schubach, Armando de O.The present study reports the production of the rabbit anti-Leishmania (L.) chagasi hyperimmune serum, the standardization of the immunohistochemistry (IHC) technique and the evaluation of its employment in cutaneous leishmaniasis (CL) lesions diagnosed by Leishmania sp. culture isolation. Thirty fragments of active CL lesions were examined as well as 10 fragments of cutaneous mycosis lesions as control group. ...