9 documents found, page 1 of 1
Olive oil waste as a source of functional food ingredients: Assessing polypheno...
Ronca, Carolina L.; Marques, Sara S.; Ritieni, Alberto; Giménez-Martínez, Rafael; Barreiros, Luísa; Segundo, Marcela A.Around two million tons of olive oil are produced in Europe annually, with Portugal being among the top five European olive oil-producing countries. Olive oil production results in a substantial amount of waste in the form of olive leaves. These discarded olive leaves contain valuable phenolic compounds with antioxidant, anti-inflammatory, hypoglycaemic, neuroprotective, and antiproliferative properties. Due to...
Microcarrier-based fluorescent yeast estrogen screen assay for fast determinati...
Gregório, Bruno J.R.; Ramos, Inês I.; Marques, Sara S.; Barreiros, Luísa; Magalhães, Luís M.; Schneider, Rudolf J.; Segundo, Marcela A.The presence of endocrine-disrupting compounds (EDCs) in water poses a significant threat to human and animal health, as recognized by regulatory agencies throughout the world. The Yeast Estrogen Screen (YES) assay is an excellent method to evaluate the presence of these compounds in water due to its simplicity and capacity to assess the bioaccessible forms/fractions of these compounds. In the presence of a com...
Exploiting kinetic features of ORAC assay for evaluation of radical scavenging ...
Carvalho, Joana R. B.; Meireles, Andreia N.; Marques, Sara S.; Gregório, Bruno J. R.; Ramos, Inês I.; Silva, Eduarda M. P.; Barreiros, LuisaThe analysis and interpretation of data retrieved from Oxygen Radical Absorbance Capacity (ORAC) assays represent a challenging task. ORAC indexes originate from different mathematical approaches often lacking correct elucidation of kinetic features concerning radical scavenging reactions by antioxidant compounds. In this work, the expression of ORAC values as area under fluorescein (FL) decay curves (AUC) and ...
Sample preparation and chromatographic methods for the determination of protein...
Fernandes, Sara R.; Meireles, Andreia N.; Marques, Sara S.; Silva, Luís; Barreiros, Luisa; Sampaio-Maia, Benedita; Miro, Manuel; Segundo, Marcela A.Protein-bound uremic retention solutes, such as indole-3-acetic acid, indoxyl sulfate, p-cresol and p-cresol sulfate, are associated with the development of several pathologies, namely renal, cardiovascular, and bone toxicities, due to their potential accumulation in the human body, thus requiring analytical methods for monitoring and evaluation. The present review addresses conventional and advanced sample tre...
Lab-on-valve automated and miniaturized assessment of nanoparticle concentratio...
Marques, Sara S.; Ramos, Inês I.; Silva, Carla; Barreiros, Luisa; Domingues, Maria R.; Segundo, Marcela A.Nanoparticles (NPs) concentration directly impacts the dose delivered to target tissues by nanocarriers. The evaluation of this parameter is required during NPs developmental and quality control stages, for setting dose−response correlations and for evaluating the reproduci bility of the manufacturing process. Still, faster and simpler procedures, dismissing skilled operators and post-analysis conversions are n...
Lab-on-valve automated and miniaturized assessment of nanoparticle concentratio...
Marques, Sara S.; Ramos, Inês I.; Silva, Carla; Barreiros, Luisa; Domingues, Maria R.; Segundo, Marcela A.Nanoparticles (NPs) concentration directly impacts the dose delivered to target tissues by nanocarriers. The evaluation of this parameter is required during NPs developmental and quality control stages, for setting dose--response correlations and for evaluating the reproducibility of the manufacturing process. Still, faster and simpler procedures, dismissing skilled operators and post-analysis conversions are n...
Insights on Ultrafiltration-Based Separation for the Purification and Quantific...
Marques, Sara S.; Ramos, Inês I.; Fernandes, Sara; Barreiros, Luisa; Lima, Sofia A. C.; Reis, Salette; Domingues, M. Rosário M.; Segundo, Marcela A.The evaluation of encapsulation efficiency is a regulatory requirement for the characterization of drug delivery systems. However, the difficulties in efficiently separating nanomedicines from the free drug may compromise the achievement of accurate determinations. Herein, ultrafiltration was exploited as a separative strategy towards the evaluation of methotrexate (MTX) encapsulation efficiency in nanostructur...
Assessment of immunoglobulin capture in immobilized protein A through automatic...
Ramos, Inês I.; Marques, Sara S.; Magalhães, Luís M.; Barreiros, Luisa; Reis, Salette; Lima, José L.F. C.; Segundo, Marcela A.The repeatable immobilization of molecular recognition elements onto particle surfaces has a strong impact on the outcomes of affinity-based assays. In this work, an automatic method for the immobilization of immunoglobulin G (IgG) onto protein A-Sepharose microbeads was established through the flow programming features of the portable lab-on-valve platform using micro-bead injection spectroscopy. The reproduci...
Fluorometric method based on molecular recognition solid-phase extraction for d...
Osório, Marcelo V.; Marques, Sara S.; Oliveira, Hugo M.; Barreiros, Luisa; Segundo, Marcela A.Riboflavin (vitamin B2) is involved in several biological processes, particularly in energy production, and it is acquired from food ingestion, principally from supplemented food during the first years of life. Therefore, a simple, fast and cost-effective high-throughput method for determination of riboflavin in milk and infant formula is proposed, based on selective extraction using commercially available mole...