76 documents found, page 1 of 8

Protein jetting-based printing: principles and applications

Protein jetting-based printing emerged as a powerful technology at the intersection of biotechnology, materials science, and microfabrication. Building on the intrinsic biocompatibility, biodegradability, and versatility of proteins, it facilitates the development of novel functional materials, by enabling precise, contactless deposition of protein-based inks onto a wide range of substrates while preserving the...

Recombinant bovine serum albumin domain II as bioreceptor for ochratoxin A capture

Established chromatographic techniques for mycotoxin control in foodstuffs require prior sample enrichment and clean-up, typically achieved using immunoaffinity columns (IACs). Bovine serum albumin (BSA) has recently emerged as a cost-effective alternative to antibodies used in IACs. This study aimed at exploring the BSA domain II (BDII), which houses the primary binding site for ochratoxin A (OTA), as a biorec...

Novel recombinant Human Serum Albumin-SPE systems for ochratoxin A determinatio...

Ochratoxin A (OTA) is a mycotoxin that raises food safety concerns in several products, requiring analytical control. Aiming to develop an alternative to immunoaffinity columns (IACs) for OTA extraction and clean-up, recombinant human serum albumin (rHSA) was explored as an affinity bioreceptor for solid-phase extraction (SPE) applications. rHSA was obtained from Pichia pastoris fused with a C-terminal His6 tag...

Microplate fluorescence quenching for high throughput screening of affinity con...

Measurements of changes in fluorescence signal is one of the most commonly applied methods for studying protein-ligand affinities. These measurements are generally carried out using cuvettes in spectrofluorometers, which can only measure one sample at a time. This makes screening procedures for multiple ligands and proteins extremely laborious, as each protein must be measured with multiple ligand concentration...

A first-in-class inhibitor of homologous recombination DNA repair counteracts t...

Background Pancreatic ductal adenocarcinoma (PDAC) is among the cancer types with poorest prognosis and survival rates primarily due to resistance to standard-of-care therapies, including gemcitabine (GEM) and olaparib. Particularly, wild-type (wt)BRCA tumours, the most prevalent in PDAC, are more resistant to DNA-targeting agents like olaparib, restraining their clinical application. Recently, we disclosed a m...

Redefining ovarian cancer treatment with a first-in-class inhibitor of homologo...

Background: High-grade serous ovarian cancer (HGSOC) is characterized by poor prognosis and low overall survival. While PARP inhibitors (PARPis) are standard maintenance therapy for BRCA-mutated HGSOC (10% of cases), acquired resis-tance develops in over 60% of patients. We identied BBIT20, a rst-in-class inhibitor of the BRCA1-BARD1 interaction that disrupts homologous recombination (HR) DNA repair. Methods: B...

Smart protein-based inks

Establishing sustainable bioproduction of lactones from metabolic engineering o...

Introduction: Lactones are volatile organic compounds derived from lipid metabolism. They have a wide range of applications, notably as fragrances and flavours, with -decalactone being particularly valuable. Lactones significantly contribute to the smell and taste of fruits like strawberries and peaches, but traditional extraction methods have many disadvantages. Microbial production has emerged as a more susta...

Recombinant proteins: design, production and applications

[Excerpt] Recombinant proteins are proteins encoded by recombinant DNA that has been cloned into an expression host. They can be manipulated in forms of existing proteins or entirely novel proteins generated by protein engineering. For instance, two or more existing proteins or peptides can be genetically fused to generate new multi-functional fusion proteins, being the use of purification/immobilization fusion...

Labelling composition, methods and uses thereof

The present disclosure relates to a labelled article comprising a substrate selected from a list consisting of at least one of the following substrates: paper, cork, leather, yarns, threads, textiles, fabrics, a particle, or combinations thereof; a reversibly switchable fluorescent peptide; and a binder peptide, preferably more than one binder, wherein the binder binds the fluorescent peptide to the substrate, ...