47 documents found, page 1 of 5
Advances in whole genome sequencing for foodborne pathogens: implications for c...
Gomes, Emílio; Araújo, Daniela; Nogueira, Teresa; Oliveira, Ricardo; Silva, Sónia Carina; Oliveira, Lorena V. N.; Azevedo, Nuno F.; Almeida, CarinaFoodborne outbreaks affecting millions of people worldwide are a significant and growing global health threat, exacerbated by the emergence of new and increasingly virulent foodborne pathogens. Traditional methods of detecting these outbreaks, including culture-based techniques, serotyping and molecular methods such as real-time PCR, are still widely used. However, these approaches often lack the precision and ...
Amphiphilic dendrimer-assisted delivery of antisense nucleic acid mimics agains...
Gomes, Mariana; Resende, Igor; Zamoshchak, Yana; Araújo, Daniela Eira; Castro, Joana Isabel Reis; Dhumal, Dinesh; Peng, Ling; Santos, Rita S.The rise in antimicrobial resistance and the consequent ineffectiveness of conventional antibiotics emphasise the need for novel therapeutic strategies. Antisense nucleic acid mimics (NAMs) are emerging as promising precision therapeutic agents, inhibiting specific genes through hybridisation with selected nucleic acid targets. However, delivering NAMs into bacteria remains a significant challenge. This study e...
Assessing host-adaptation of new sequence types of livestock-associated methici...
Oliveira, Ricardo; Araújo, Daniela; Castro, Joana Isabel Reis; Nogueira, Teresa; Almeida, Gonçalo; Azevedo, Nuno F.; Almeida, CarinaThe importance of animals as reservoirs of human pathogens is widely recognised, with livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) being consistently reported. This study presents a phenotypic and genotypic characterization of five MRSA isolates from raw bovine milk in northern Portugal, using culturomics and whole genome sequencing (WGS), to assess their potential risk for human i...
Advances in whole genome sequencing for foodborne pathogens: implications for c...
Gomes, Emílio; Araújo, Daniela; Nogueira, Teresa; Oliveira, Ricardo; Silva, Sónia; Oliveira, Lorena V. N.; Azevedo, Nuno F.; Almeida, CarinaFoodborne outbreaks affecting millions of people worldwide are a significant and growing global health threat, exacerbated by the emergence of new and increasingly virulent foodborne pathogens. Traditional methods of detecting these outbreaks, including culture-based techniques, serotyping and molecular methods such as real time PCR, are still widely used. However, these approaches often lack the precision andr...
Exploring the potential of cell-free supernatants in the control of foodborne p...
Ferreira, Sara; Gomes, Emílio; Araújo, Daniela Eira; Azevedo, Nuno F.; Almeida, Carina Manuela Fernandes; Castro, Joana Isabel Reis[Excerpt] Foodborne pathogens are a constant public health concern, frequently caus ing outbreaks that can lead to severe consequences. Traditional methods for controlling these microorganisms, such as antibiotics and disinfectants, are becoming less effective due to the rise in microbial resistance. Excessive use of these substances can contribute to the development of resistant strains, making it harder to ma...
PLGA nanoparticles double-decorated with a TAT peptide and folic acid to target...
Andrade, Stéphanie; Ramalho, Maria J.; Santos, João; Santos, Sílvio Roberto Branco; Melo, Luís Daniel Rodrigues; Guimarães, Nuno; Ferraz, Maria P.Treating bacterial infections has become increasingly difficult due to the rise in antibiotic-resistant bacterial strains. Strategies involving the targeted delivery of antibiotics have been proposed to minimize the administered antibiotic doses. This study aims to develop the first double-modified nanovehicle capable of increasing bacterial membranes permeability while specifically targeting Staphylococcus aur...
Avaliação do potencial de sobrenadantes livres de células (SLC) contra agentes ...
Ferreira, Sara; Gomes, Emílio; Araújo, Daniela; Azevedo, Nuno F.; Almeida, Carina Manuela Fernandes; Castro, JoanaOs agentes patogénicos de origem alimentar são um problema recorrente de saúde pública, causando surtos frequentes que podem ter consequências drásticas. De modo a combater estes microrganismos, diversas medidas são habitualmente aplicadas, nomeadamente o uso de antibióticos e desinfetantes. No entanto, o uso excessivo destas substâncias pode levar ao desenvolvimento de resistência ou tolerância por parte dos m...
The role of flagellum and flagellum-based motility on Salmonella enteritidis an...
Vilas Boas, Diana; Castro, Joana Isabel Reis; Araújo, Daniela; Nóbrega, Franklin L.; Keevil, Charles W.; Azevedo, Nuno F.; Vieira, Maria JoãoFlagellum-mediated motility has been suggested to contribute to virulence by allowing bacteria to colonize and spread to new surfaces. In Salmonella enterica and Escherichia coli species, mutants affected by their flagellar motility have shown a reduced ability to form biofilms. While it is known that some species might act as co-aggregation factors for bacterial adhesion, studies of food-related biofilms have ...
The potential of tailed amplicons for SARS-CoV-2 detection in nucleic acid late...
Vindeirinho, João M.; Oliveira, Ricardo; Pinho, Eva; Guiomar, Raquel; Azevedo, Nuno F.; Almeida, Carina Manuela FernandesNucleic Acid Lateral Flow Assays (NALFAs) are a promising solution for the point-of-care detection of viruses like SARS-CoV-2. However, they show some drawbacks, such as the great dependency on the use of antibodies and the need for post-amplification protocols that enable the preparation of amplicons for effective readings, as well as low sensitivity. Here, we developed amplicons of a specific SARS-CoV-2 gene ...
Advances on diagnosis of Helicobacter pylori infections
Sousa, Cláudia Sofia Cunha; Ferreira, Rute Vanessa Novais; Santos, Sílvio Roberto Branco; Azevedo, Nuno F.; Melo, Luís Daniel RodriguesThe association of Helicobacter pylori to several gastric diseases, such as chronic gastritis, peptic ulcer disease, and gastric cancer, and its high prevalence worldwide, raised the necessity to use methods for a proper and fast diagnosis and monitoring the pathogen eradication. Available diagnostic methods can be classified as invasive or non-invasive, and the selection of the best relies on the clinical cond...