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Long non-coding RNAs from Bursaphelenchus xylophilus involved in the interactio...

Mendonça, Madalena; Vicente, Cláudia S. L.; Espada, Margarida

Pinewood nematode (PWN), B. xylophilus, is a migratory endoparasite that causes major forestry losses worldwide. Due to their function as key regulators of different biological processes, long non-coding RNAs (lncRNA) can be involved in nematode virulence regulation, adaptation and pathogenesis. In this study, we aimed to characterized PWN lncRNAs expression and understand their potential function in parasitism...


A importância dos nemátodes fitoparasitas na sustentabilidade dos citrinos em P...

Barbosa, Pedro; Mendonça, Madalena; Gutiérrez-Gutiérrez, Carlos; Espada, Margarida; Vicente, Cláudia S. L.

Os nemátodes fitoparasitas (NFP) representam uma ameaça às culturas agrícolas em todo o mundo, causando uma diminuição do seu rendimento e qualidade, bem como um aumento dos custos de produção. Existem, aproximadamente, 4000 espécies de NFP e todas as plantas cultivadas são suscetíveis a pelo menos uma destas espécies. Globalmente são conhecidas quatro espécies de NFP que afetam os citrinos - Tylenchulus semipe...


A importância dos nemátodes fitoparasitas na sustentabilidade da viticultura

Barbosa, Pedro; Mendonça, Madalena; Espada, Margarida; Vicente, Cláudia S. L.

Os nemátodes fitoparasitas são animais microscópicos, vermifor- mes, que se alimentam maioritariamente das raízes das plantas, reduzindo o seu vigor e produtividade. São responsáveis por per- das económicas expressivas em diferentes culturas agronómicas e apresentam, de um modo geral, uma distribuição mundial e adaptação a diferentes condições edafoclimáticas.


Identification of parasitism-related genes in the specialized esophageal gland ...

Mendonça, Madalena; Vicente, Claudia S.L.; Espada, Margarida

The pinewood nematode (PWN), Bursaphelenchus xylophilus, is a migratory plant-parasitic nematode responsible for the pine wilt disease (PWD), causing economic and ecological losses in the forestry industry. Current climate change predictions emphasize an increase of PWD on conifers worldwide. The absence of effective measures to control PWN and the present restrictions on the use of chemical pesticides require ...


Functional characterization of ShK domain-containing protein in the plant- para...

Mendonça, Madalena; Vicente, Cláudia S. L.; Espada, Margarida

Plant pathogens cause severe economic and ecological problems in a wide range of crops and forestry plant species worldwide. The migratory plant parasitic nematode Bursaphelenchus xylophilus (pinewood nematode, PWN) is a European quarantine organism which causes economic and ecological damage to the forestry industry leading to the death of the tree. The interactions of plant-parasitic nematodes with their host...


Functional characterization of ShK domain-containing protein in the plant-paras...

Mendonça, Madalena; Vicente, Cláudia; Espada, Margarida

ShK domain-containing proteins are peptides found in different parasitic and venomous organisms. From a previous transcriptomic dataset from Bursaphelenchus xylophilus, a plant-endoparasitic nematode that infects forest tree species, we identified 96 transcripts potentially as ShK domain-containing proteins with unknown function in the nematode genome. This study aimed to characterize and explore the functional...


Developing sustainable approaches for the control of plant- parasitic nematodes...

Barbosa, Pedro; Mendonça, Madalena; Costa, Marina; Gutiérrez-Gutiérrez, Carlos; Mota, Manuel; Espada, Margarida; Vicente, Cláudia S. L.

Plant-parasitic nematodes (PPN) are a serious threat for economically important crops worldwide, severely affecting the sustainability of the agricultural and forestry systems. Some PPNs are listed as quarantine pests for which restrictive regulations are imposed by phytosanitary authorities. Preventive control management strategies can help in the early detection of these pathogens. At Nemalab (Laboratory of N...


How parasitism genes are regulated: a motif to search for genes regulators in t...

Mendonça, Madalena; Vicente, Cláudia S. L.; Barbosa, Pedro; Velada, Isabel; Vieira, Paulo; Faria, Jorge; Santos, Margarida; Nóbrega, Filomena

Plant-parasitic nematodes threaten global agricultural and forestry systems. The search for new control strategies in line with the EU’s sustainability goals highlight significant knowledge gaps. Like all other plant pathogens, plant-parasitic nematodes deliver several parasitism proteins (effectors) into the host plant to cause disease. NemaWAARS project focuses on mechanism(s) of regulation and gene control e...


Parasitismo no nemátode da madeira do pinheiro – Estudo dos mecanismos de regul...

Espada, Margarida; Mendonça, Madalena; Mota, Manuel; Inácio, Maria de Lurdes

A Fileira do Pinho representou, em 2020, 80% dos postos de trabalho e 45% do volume de negócios nas indústrias florestais, assim como mais de 3% das exportações nacionais de bens. Há, no entanto, um défice de 2,3Mm3 de madeira de pinho que é justificado pelo fogo e a presença do nemátode da madeira do pinheiro (NMP), Bursaphelenchus xylophilus (Centro PINUS, 2021) (Figura 1). A doença da murchidão do pinheiro, ...


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