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Molecular characterization reveals Brazilian Tomato chlorosis virus to be close...
Albuquerque, Leonardo Cunha de; Villanueva, Francisco; Resende, Renato O.; Navas-Castillo, Jesús; Barbosa, Júlio César; Nagata, Alice Kazuko InoueTomato chlorosis virus (ToCV, genus Crinivirus, family Closteroviridae) is a whitefly-transmitted crinivirus with a bipartite RNA genome. This virus is emerging as a serious threat to tomato crops worldwide. To date, only three complete genomic sequences of ToCV have been described from North America, Spain, and Greece isolates. In this study, we present the fourth complete nucleotide sequence of the RNA 1 (859...
Detection and complete genome characterization of a begomovirus infecting okra ...
Aranha, Silvia de Araujo; Albuquerque, Leonardo Cunha de; Boiteux, Leonardo Silva; Nagata, Alice Kazuko InoueA survey of okra begomoviruses was carried out in Central Brazil. Foliar samples were collected in okra production fields and tested by using begomovirus universal primers. Begomovirus infection was confirmed in only one (#5157) out of 196 samples. Total DNA was subjected to PCR amplification and introduced into okra seedlings by a biolistic method; the bombarded DNA sample was infectious to okra plants. The DN...
Development of a species-specific detection method for three Brazilian tomato b...
Fernandes, Fernanda Rausch; Albuquerque, Leonardo Cunha de; Nagata, Alice Kazuko InoueThe begomoviruses (fam. Geminiviridae) have an ssDNA genome, are transmitted by whiteflies, and cause significant losses in tomato fields in Brazil. Nowadays, begomovirus species identification is carried out through the analysis of their complete genome sequence. Due to the high costs and difficulty in applying this technique to large-scale analysis, we aimed to develop a species-specific detection method base...
Diversidade de begomovírus mono e bipartidos infectando tomateiro (solanum lyco...
Albuquerque, Leonardo Cunha deTese (doutorado)—Universidade de Brasília, Instituto de Ciências Biológicas, Departamento de Fitopatologia, Programa de Pós-Graduação em Fitopatologia, 2012.; Os begomovírus (família Geminiviridae, gênero Begomovirus) possuem genoma constituído por DNA circular de fita simples, apresentam um (monopartido) ou dois (bipartido, DNA-A e DNA-B) componentes genômicos e são transmitidos naturalmente a diversas espécie...
Caracterização molecular e biológica de um isolado de Tomato yellow vein streak...
Albuquerque, Leonardo Cunha deDissertação (mestrado)—Universidade de Brasília, Instituto de Ciências Biológicas, Departamento de Fitopatologia, 2008.; A família Geminiviridae é caracterizada pela morfologia geminada das partículas e genoma composto por DNA circular de fita simples. Esta família é dividida em quatro gêneros, Mastrevirus, Begomovirus, Curtovirus e Topocuvirus, de acordo com a espécie de inseto vetor, círculo de hospedeiros e ...