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Effects of early-life amino acids supplementation on fish responses to a therma...

Navarro-Guillén, Carmen; Jerez-Cepa, Ismael; D. Lopes, André; Mancera, Juan Miguel; Engrola, Sofia

Nutritional programming is a promising concept for promoting metabolic adaptation of fish to challenging conditions, such as the increase in water temperature. The present work evaluates in ovo arginine or glutamine supplementation as enhancers of zebrafish metabolic or absorptive capacity, respectively, at optimum (28 degrees C) and challenging temperatures (32 degrees C) in the long-term. Growth performance, ...


Effects of dietary curcumin in growth performance, oxidative status and gut mor...

Xavier, Maria João; Navarro-Guillén, Carmen; Lopes, André; Colen, R.; Teodósio, Rita; Mendes, Rodrigo; Oliveira, Beatriz; Valente, Luisa M.P.

An imbalance in the production and detoxification of reactive oxygen species and other oxidants can disrupt a l l types of cellular compounds, and lead to a state of oxidative stress. Preventing this state is essential to improve growth and health in animal production. Therefore, the aim of this work was to assess i f the dieta r y supple-mentation of curcumin cou l d improve the antioxidant status and intestin...


Dietary curcumin promotes gilthead seabream larvae digestive capacity and modul...

Xavier, Maria João; Dardengo, Gian Marco; Navarro-Guillén, Carmen; Lopes, Andre D.; Colen, R.; Valente, Luisa M. P.; Conceição, Luís E. C.

The larval stage is highly prone to stress due to the ontogenetic and metabolic alterations occurring in fish. Curcumin inclusion in diets has been shown to improve growth by modulating oxidative status, immune response, and/or feed digestibility in several fish species. The aim of the present work was to assess if dietary curcumin could promote marine fish larvae digestive maturation and improve robustness. Gi...


Replacement of Antarctic krill (Euphausia superba) by extruded feeds with diffe...

Goncalves, Renata; Gesto, Manuel; Teodosio, Maria; Baptista, Vânia; Navarro-Guillén, Carmen; Lund, Ivar

Extruded feeds are widely used for major aquatic animal production, particularly for finfish. However, the transition from fresh/frozen to extruded/pelleted feeds remains a major obstacle to progressing sustainable farming of European lobster ( Homarus gammarus). The aim of the present study was to investigate the effects of using extruded feeds with different protein levels and lipid/carbohydrate ratios on gro...


Ontogeny and diurnal patterns of molecular gene expression and activity of dige...

Gamberoni, P.; Yufera, M.; de las Heras, V; Siguero, I; Gilannejad, N.; Martinez-Rodriguez, G.; Navarro-Guillén, Carmen

The greater amberjack, Seriola dumerili, is a fast-growing pelagic teleost with great interest for the diversification of farmed marine fish species in the Mediterranean region. Aiming to advance the optimization of feeding during the early stage of this species, this study examines the ontogeny of digestive function using molecular and biochemical approaches. Gene expression of digestive enzyme precursors and ...


Does a ghrelin stimulus during zebrafish embryonic stage modulate its performan...

Navarro-Guillén, Carmen; Dias, Jorge; Rocha, Filipa; Castanheira, M. F.; Martins, Catarina I. M.; Laizé, Vincent; Gavaia, Paulo; Engrola, Sofia

Metabolic programming refers to the induction, deletion, or impaired development of a somatic structure or "setting" of a physiological system by an early life stimulus operated at a critical period during development. Ghrelin is the only known orexigenic gut hormone, is an acylated peptide that acts as an endogenous ligand specific for growth-hormone secretagogue-receptor. The aim of the present work was to ev...


Effect of increased rearing temperature on digestive function in cobia early ju...

Yúfera, M.; Nguyen, M.V.; Navarro-Guillén, Carmen; Moyano, F.J.; Jordal, A.-E.O.; Espe, M.; Conceição, L.E.C.; Engrola, Sofia; Le, M.H.; Rønnestad, I.

The present study is focused to elucidate the main characteristics of the digestive function of this carnivorous fast-growing fish living at high temperatures. With this aim, we have examined the effects of an increased temperature from 30 to 34 °C on the daily pattern of gastrointestinal pH, enzymatic proteolytic digestive activity and the feed transit time in early juveniles of cobia (Rachycentron canadum), a...


Fast growing greater amberjack post-larvae require a high energy-high protein w...

Navarro-Guillén, Carmen; Conceicao, L. E. C.; Pinto, W.; Siguero, I; Urrutia, P.; Moyano, F. J.; Yufera, M.

Larvae and early juveniles of fast-growing fish species show tremendous growth potential, leading to higher requirements for protein, high-unsaturated fatty acids, and other nutrients. Several commercial weaning diets with relatively high success have been developed for low/moderate-growing species however, additional challenges are outlined to meet growth potential and energy requirements of such fast-growing ...


Ghrelin in Senegalese sole (Solea senegalensis) post-larvae: Paracrine effects ...

Navarro-Guillén, Carmen; Yufera, Manuel; Engrola, Sofia

Successful food consumption and digestion depend on specifics anatomical and behavioral characteristics and corresponding physiological functions that should be ready to work at the appropriate time. The physiological regulation of appetite and ingestion involves a complex integration of peripheral and central signals by the brain. Ghrelin is a peptide hormone involved in the control of energy homeostasis and i...


Effect of varying dietary levels of LC-PUFA and vegetable oil sources on perfor...

Navarro-Guillén, Carmen; Engrola, S.; Castanheira, Maria Filipa; Bandarra, N.; Hachero-Cruzado, Ismael; Tocher, Douglas R.; Conceição, L. E. C.

Lipid nutrition of marine fish larvae has focused on supplying essential fatty acids (EFA) at high levels to meet requirements for survival, growth and development. However, some deleterious effects have been reported suggesting that excessive supply of EFA might result in insufficient supply of energy substrates, particularly in species with lower EFA requirements such as Senegalese sole (Solea senegalensis). ...


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