26 documents found, page 1 of 3
Differential effects of the essential oils of Lavandula luisieri and Eryngium d...
Rufino, AT; Ferreira, I; Judas, F; Salgueiro, L; Lopes, MC; Cavaleiro, C; Mendes, AFCONTEXT: Effective drugs to treat osteoarthritis (OA) and inflammatory bowel disease (IBD) are needed. OBJECTIVE: To identify essential oils (EOs) with anti-inflammatory activity in cell models of OA and IBD. MATERIALS AND METHODS: EOs from Eryngium duriaei subsp. juresianum (M. Laínz) M. Laínz (Apiaceae), Laserpitium eliasii subsp. thalictrifolium Sennen & Pau (Apiaceae), Lavandula luisieri (Rozeira) Rivas-Mar...
Treatment planning systems dosimetry auditing project in Portugal
Lopes, MC; Cavaco, A; Jacob, K; Madureira, L; Germano, S; Faustino, S; Lencart, J; Trindade, M; Vale, J; Batel, V; Sousa, M; Bernardo, A; Brás, SBACKGROUND AND PURPOSE: The Medical Physics Division of the Portuguese Physics Society (DFM_SPF) in collaboration with the IAEA, carried out a national auditing project in radiotherapy, between September 2011 and April 2012. The objective of this audit was to ensure the optimal usage of treatment planning systems. The national results are presented in this paper. MATERIAL AND METHODS: The audit methodology simu...
Treatment planning systems dosimetry auditing project in Portugal
Lopes, MC; Cavaco, A; Jacob, K; Madureira, L; Germano, S; Faustino, S; Lencart, J; Trindade, M; Vale, J; Batel, V; Sousa, M; Bernardo, A; Brás, SBACKGROUND AND PURPOSE: The Medical Physics Division of the Portuguese Physics Society (DFM_SPF) in collaboration with the IAEA, carried out a national auditing project in radiotherapy, between September 2011 and April 2012. The objective of this audit was to ensure the optimal usage of treatment planning systems. The national results are presented in this paper. MATERIAL AND METHODS: The audit methodology simu...
Expression and function of K(ATP) channels in normal and osteoarthritic human c...
Rufino, AT; Rosa, SC; Judas, F; Mobasheri, A; Lopes, MC; Mendes, AF
Expression and function of K(ATP) channels in normal and osteoarthritic human c...
Rufino, AT; Rosa, SC; Judas, F; Mobasheri, A; Lopes, MC; Mendes, AFATP-sensitive potassium [K(ATP)] channels sense intracellular ATP/ADP levels, being essential components of a glucose-sensing apparatus in various cells that couples glucose metabolism, intracellular ATP/ADP levels and membrane potential. These channels are present in human chondrocytes, but their subunit composition and functions are unknown. This study aimed at elucidating the subunit composition of K(ATP) ch...
Amplified and Homozygously Deleted Genes in Glioblastoma: Impact on Gene Expres...
Crespo, I; Tão, H; Nieto, AB; Rebelo, O; Domingos, P; Vital, AL; Patino, MC; Barbosa, MD; Lopes, MC; Resende de Oliveira, C; Orfão, A; Tabernero, MDBACKGROUND: Glioblastoma multiforme (GBM) displays multiple amplicons and homozygous deletions that involve relevant pathogenic genes and other genes whose role remains unknown. METHODOLOGY: Single-nucleotide polymorphism (SNP)-arrays were used to determine the frequency of recurrent amplicons and homozygous deletions in GBM (n = 46), and to evaluate the impact of copy number alterations (CNA) on mRNA levels of...
Screening of five essential oils for identification of potential inhibitors
Neves, A; Rosa, S; Gonçalves, J; Rufino, A; Judas, F; Salgueiro, L; Lopes, MC; Cavaleiro, C; Mendes, AFNuclear factor-kappaB is a key transcription factor activated by pro-inflammatory signals, like interleukin-1beta (IL-1), being required for the expression of many inflammatory and catabolic mediators, such as nitric oxide (NO), that play an important role in arthritic diseases. This work aimed at screening and identifying natural inhibitors of IL-induced NF-kappaB activation and NO production in human articula...
Signal transduction profile of chemical sensitisers in dendritic cells: an endp...
Neves, BM; Gonçalo, Margarida; Figueiredo, A; Duarte, CB; Lopes, MC; Cruz, MTThe development of non-animal testing methods for the assessment of skin sensitisation potential is an urgent challenge within the framework of existing and forthcoming legislation. Efforts have been made to replace current animal tests, but so far no alternative methods have been developed. It is widely recognised that alternatives to animal testing cannot be accomplished with a single approach, but rather wil...
Expression and function of the insulin receptor in normal and osteoarthritic hu...
Rosa, SC; Rufino, AT; Judas, F; Tenreiro, C; Lopes, MC; Mendes, AFOBJECTIVE: Chondrocytes respond to insulin, but the presence and role of the specific high affinity insulin receptor (InsR) has never been demonstrated. This study determined whether human chondrocytes express the InsR and compared its abundance and function in normal and osteoarthritis (OA) human chondrocytes. DESIGN: Cartilage sections were immunostained for detection of the InsR. Non-proliferating chondrocyt...
Role of glucose as a modulator of anabolic and catabolic gene expression in nor...
Rosa, SC; Rufino, AT; Judas, F; Tenreiro, CM; Lopes, MC; Mendes, AFCartilage matrix homeostasis involves a dynamic balance between numerous signals that modulate chondrocyte functions. This study aimed at elucidating the role of the extracellular glucose concentration in modulating anabolic and catabolic gene expression in normal and osteoarthritic (OA) human chondrocytes and its ability to modify the gene expression responses induced by pro-anabolic stimuli, namely Transformi...