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Genetic variability of papaya lethal yellowing virus isolates from Ceará and Ri...
Pereira, Álvaro J.; Cascardo, Renan S.; Alfenas-Zerbini, Poliane; Bezerra-Junior, José Evando A.; Lima, José Albérsio A.; Zerbini, Francisco MuriloO mamão (Carica papaya) é uma fruta de grande importância econômica em todo o Nordeste brasileiro, região responsável por 60% da produção nacional. Mamoeiros nos estados do Ceará e Rio Grande do Norte são afetados pela doença denominada amarelo letal do mamoeiro, causada pelo papaya lethal yellowing virus (PLYV). Trabalhos anteriores indicaram que o PLYV é um possível sobemovírus. A fim de estimar a variabilida...
Genomic and biological characterization of a new member of the genus Phikmvviru...
Xavier, André da Silva; Silva, Fernanda Pereira da; Vidigal, Pedro Marcus Pereira; Lima, Thamylles Thuany Mayrink; Souza, Flavia Oliveira deBacterial wilt caused by Ralstonia spp., soil-borne Gram-negative bacteria, is considered one of the most important plant diseases in tropical and subtropical regions of the world. A large number of bacteriophages capable of lysing or physiologically reprogramming cells of Ralstonia spp. have been reported in Asia. Despite the potential use of these organisms in the management of bacterial wilt, information on ...
Six novel begomoviruses infecting tomato and associated weeds in Southeastern B...
Castillo-Urquiza, Gloria P.; Beserra Jr., José Evando A.; Bruckner, Fernanda P.; Lima, Alison T. M.; Varsani, Arvind; Alfenas-Zerbini, PolianeThe incidence of tomato-infecting begomoviruses has sharply increased in Brazil following the introduction of the B biotype of the whitefly vector in the early 1990s. Five definitive species and six tentative species have been described since then. Here, we report the detection of members of an additional six novel species, three in tomato and three infecting weeds that are commonly associated with tomato field...
Comparative analysis of the genomes of two isolates of cowpea aphid-borne mosai...
Barros, Danielle R.; Alfenas-Zerbini, Poliane; BeserraJr., José Evando A.; Antunes, Tathiana F. S.; Zerbini, F. MuriloThe complete genomic sequences of two cowpea aphid-borne mosaic virus (CABMV) isolates from Brazil, MG-Avr from passion fruit (which also infects cowpea), and BR1 from peanut (which also infects cowpea, but not passion fruit), were determined. Their nucleotide sequences are 89% identical and display 85% identity to that of CABMV-Z. Both isolates have the typical potyvirus genome features. P3 and VPg are the mos...
A novel mycovirus associated to Alternaria alternata comprises a distinct linea...
Xavier, André da Silva; Barros, Ana Paula Oliveira de; Godinho, Márcio Tadeu; Zerbini, Francisco Murilo; Souza, Flávia de OliveiraIn the present work, we report a novel mycovirus that infects Alternaria alternata. The mycovirus has isometric particles of approximately 30 nm and the genome consists of two molecules of dsRNA, dsRNA1 with 1833 bp, encoding a putative RNA-dependent RNA polymerase (RdRp) and dsRNA2, with 1680 bp in length, encoding the putative capsid protein (CP). RdRp analysis revealed low amino acid identity with RdRps with...
Analysis of the full-length genome sequence of papaya lethal yellowing virus (P...
Pereira, Álvaro J.; Alfenas-Zerbini, Poliane; Cascardo, Renan S.; Andrade, Eduardo C.; Zerbini, F. MuriloPapaya lethal yellowing virus (PLYV) causes an economically important disease in papayas in northeastern Brazil. Based on biological and molecular properties, PLYV has been tentatively assigned to the genus Sobemovirus. We report the sequence of the full-length genome of a PLYV isolate from Brazil, determined by deep sequencing. The PLYV genome is 4,145 nt long and contains four ORFs, with an arrangement identi...
Synergism and negative interference during co-infection of tomato and Nicotiana...
Alves-Júnior, Miguel; Alfenas-Zerbini, Poliane; Andrade, Eduardo C.; Esposito, Débora A.; Silva, Fábio N.; Cruz, Ana Cláudia F. daIn Brazil, at least eight begomoviruses including Tomato rugose mosaic virus (ToRMV) and Tomato yellow spot virus (ToYSV) infect tomatoes. ToYSV symptoms in tomato and Nicotiana benthamiana appear earlier and are more severe compared to those of ToRMV. We investigated the role of several factors in this differential adaptation. To analyze infection kinetics, a single leaf was inoculated and subsequently detache...
Genome-Wide analysis of differentially expressed genes during the early stages ...
Alfenas-Zerbini, Poliane; Maia, Ivan G.; Fávaro, Regiane D.; Cascardo, Julio C. M.; Brommonschenkel, Sérgio H.; Zerbini, Francisco MuriloPlant responses against pathogens cause up- and downward shifts in gene expression. To identify differentially expressed genes in a plant-virus interaction, susceptible tomato plants were inoculated with the potyvirus Pepper yellow mosaic virus (PepYMV) and a subtractive library was constructed from inoculated leaves at 72 h after inoculation. Several genes were identified as upregulated, including genes involv...
Brazilian begomovirus populations are highly recombinant, rapidly evolving, and...
Rocha, Carolina S.; Castillo-Urquiza, Gloria P.; Lima, Alison T. M.; Silva, Fábio N.; Xavier, Cesar A. D.; Hora-Júnior, Braz T.The incidence of begomovirus infections in crop plants sharply increased in Brazil during the 1990s following the introduction of the invasive B biotype of the whitefly vector, Bemisia tabaci. It is believed that this biotype transmitted begomoviruses from noncultivated plants to crop species with greater efficiency than indigenous B. tabaci biotypes. Either through rapid host adap- tation or selection pressure...
Sustained NIK-mediated antiviral signalling confers broad-spectrum tolerance to...
Brustolini, Otávio J.B.; Machado, Joao Paulo B.; Condori-Apfata, Jorge A.; Coco, Daniela; Deguchi, Michihito; Loriato, Virgílio A.P.Begomovirus-associated epidemics currently threaten tomato production worldwide due to the emergence of highly pathogenic virus species and the proliferation of a whitefly B biotype vector that is adapted to tomato. To generate an efficient defence against begomovirus, we modulated the activity of the immune defence receptor nuclear shuttle protein (NSP)-interacting kinase (NIK) in tomato plants; NIK is a virul...