23 documents found, page 1 of 3
Bioprospecting bioactive peptides in Halobatrachus didactylus Body Mucus: From ...
Cunha, Marta Fernandez; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, Maria ManuelaFish body mucus plays a protective role, especially in Halobatrachus didactylus, which inhabits intertidal zones vulnerable to anthropogenic contaminants. In silico predicted bioactive peptides were identified in its body mucus, namely, EDNSELGQETPTLR (HdKTLR), DPPNPKNL (HdKNL), PAPPPPPP (HdPPP), VYPFPGPLPN (HdVLPN), and PFPGPLPN (HdLPN). These peptides were studied in vitro for bioactivities and aggregation be...
Bioprospecting bioactive peptides in halobatrachus didactylus body mucus: from ...
Cunha, Marta Fernandez; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, Maria ManuelaFish body mucus plays a protective role, especially in Halobatrachus didactylus, which inhabits intertidal zones vulnerable to anthropogenic contaminants. In silico predicted bioactive peptides were identified in its body mucus, namely, EDNSELGQETPTLR (HdKTLR), DPPNPKNL (HdKNL), PAPPPPPP (HdPPP), VYPFPGPLPN (HdVLPN), and PFPGPLPN (HdLPN). These peptides were studied in vitro for bioactivities and aggregation be...
Southernmost record of Liparis montagui (Donovan, 1804) (Perciformes, Liparidae...
Duarte-Coelho, Pedro; Miranda, Carolina; Pereira, Ana; Vasco-Rodrigues, Nuno; Almada, FredericoIn January 2022, the presence of adult <jats:italic>Liparis montagui (Donovan, 1804) was documented at its southernmost point along the west coast of Portugal. The species was identified through both morphological and genetic barcoding analyses. This observation, part of an ongoing long-term coastal survey, suggests that L. montagui may be a transient species, influenced by complex climate and oceanic interacti...
Bioaccessibility of novel bioactive peptides from the body mucus of the Lusitan...
Fernandez Cunha, Marta; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, ManuelaThe bioprospection of marine resources for drug discovery is receiving increasing attention (1). Adverse marine environmental conditions lead organisms to develop a collection of bioactive molecules for survival (1). Mucus, acting as a first line of defense against pathogens (2), is known to protect fish from the surrounding environment. Our previous studies have already demonstrated the bioactive potential of ...
NanoLC-MS/MS sequencing and in silico analysis of bioactive peptides from Lusit...
Cunha, Marta Fernandez; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, ManuelaThe mucus covering a fish's body works as a protective barrier and contains molecules, such as antimicrobial peptides, that defend against pathogens. In this study, two adult Halobatrachus didactylus were captured from the wild in Sesimbra, and mucus was collected by scraping the dorsal-lateral body with a sponge. Our objective was to identify new bioactive peptides in mucus samples via chromatography. SE-HPLC ...
Sustainable bioprospecting and circular economy approach: fractionation and ide...
Cunha, Marta Fernandez; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, ManuelaBioactive molecules from marine organisms, especially the Halobatrachus didactylus, also known as the Lusitanian toadfish, native to the Portuguese coast, remains largely unexplored. It is important to use sustainable bioprospecting practices to protect both the species and its ecosystem (1). Therefore, by adopting a circular economy approach, we can maximize the sustainable use of marine resources by sampling ...
Bioaccessibility of novel bioactive peptides from the body mucus of the Lusitan...
Fernandez Cunha, Marta; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, ManuelaThe bioprospection of marine resources for drug discovery is receiving increasing attention (1). Adverse marine environmental conditions lead organisms to develop a collection of bioactive molecules for survival (1). Mucus, acting as a first line of defense against pathogens (2), is known to protect fish from the surrounding environment. Our previous studies have already demonstrated the bioactive potential of ...
NanoLC-MS/MS sequencing and in silico analysis of bioactive peptides from Lusit...
Cunha, Marta Fernandez; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, ManuelaThe mucus covers the fish's body, working as a protective barrier. Besides physical protection, mucus provides molecules that protect the fish from damaging pathogens [1,2]. Has been reported that antimicrobial peptides are secreted in the mucus, which play an essential role in defense against microbial pathogens since these belong to the innate immune system [2,3].
Methods for the collection of fish mucus: a systematic review
Cunha, Marta Fernandez; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, ManuelaThe aquatic environment holds a vast source of organisms that provide numerous opportunities to bioprospect new molecules. Notably, fish are producers of an epidermal mucus that offers protection against pathogens, making it a promising source of bioactive molecules. This source of molecules, however, has yet to be thoroughly explored, and particularly, optimization of methods for collection and study is needed...
Bioactive potential and chromatographic characterization of body mucus from Por...
Cunha, Marta; Coscueta, Ezequiel R.; Brassesco, María Emilia; Almada, Frederico; Gonçalves, David; Pintado, ManuelaThe body mucus in fish provides a stable physical or chemical barrier against invading pathogens. In mucus, antimicrobial peptides are secreted as a response to immunestimulation. Studies have shown antimicrobial activity against multidrugresistant pathogens and low toxicity to eukaryotic cells. Previously, body mucus from five captive Halobatrachus didactylus individuals was collected. We aim to explore the m...