29 documents found, page 1 of 3
The green generation of sunscreens: Using coffee industrial sub-products
Marto, J.; Gouveia, L. F.; Chiari, B. G. [UNESP]; Paiva, A.; Isaac, V. [UNESP]; Pinto, P.; Simões, P.; Almeida, A. J.; Ribeiro, H. M.Made available in DSpace on 2018-12-11T16:39:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-02-01; Spent coffee grounds and green coffee defective beans, which are industrial sub-products of coffee processing, have a potential use for cosmetic applications, due to their safety and high content in lipids that present interesting physicochemical properties. Sunscreen formulations in the form of water-i...
Design of novel starch-based Pickering emulsions as platforms for skin photopro...
Marto, J.; Gouveia, L. F.; Gonçalves, L.; Chiari-Andréo, B. G. [UNESP]; Isaac, V. [UNESP]; Pinto, P.; Oliveira, E.; Almeida, A. J.; Ribeiro, H. M.Made available in DSpace on 2018-12-11T16:42:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-09-01; Fundação para a Ciência e a Tecnologia; Green coffee oil and modified starch were recently found to have an enhanced protection effect against UV radiation. Therefore, this work aimed to develop an innovative sunscreen formulation based on Pickering emulsions concept, i.e., surfactant-free emulsions sta...
Morphological heterogeneity of Paracoccidioides brasiliensis: relevance of the ...
Menino, João F.; Osório, Nuno S.; Sturme, Mark H. J.; Barros, Diana Cláudia Martins Costa; Alves, Ana G. Gomes; Almeida, A. J.; Costa, Patrício SoaresParacoccidioides brasiliensis budding pattern and polymorphic growth were previously shown to be closely linked to the expression of PbCDC42 and to infl uence the pathogenesis of the fungus. In this work we conducted a detailed morphogenetic evaluation of the yeast-forms of 11 different clinical and environmental P. brasiliensis isolates comprising four phylogenetic lineages (S1, PS2, PS3 and Pb01-like), as wel...
The rs5743836 polymorphism in TLR9 confers a population-based increased risk of...
Carvalho, Agostinho; Cunha, Cristina; Almeida, A. J.; Osório, Nuno S.; Saraiva, Margarida; Coelho, Maria Teixeira; Pedreiro, Susana; Torrado, EgídioNon-Hodgkin lymphoma (NHL) has been associated with immunological defects, chronic inflammatory and autoimmune conditions. Given the link between immune dysfunction and NHL, genetic variants in toll-like receptors (TLRs) have been regarded as potential predictive factors of susceptibility to NHL. Adequate anti-tumoral responses are known to depend on TLR9 function, such that the use of its synthetic ligand is b...
Gene knockdown in Paracoccidioides brasiliensis using antisense RNA
Menino, João F.; Almeida, A. J.; Rodrigues, Fernando José dos SantosParacoccidioides brasiliensis is a thermal dimorphic fungus which at host environment exhibits a multinucleated and multibudding yeast form. The cellular and molecular mechanisms underlying these phenotypes remain to be clarified, mostly due to the absence of efficient classical genetic and molecular techniques. Here we describe a method for gene expression knockdown in P. brasiliensis by antisense RNA (aRNA) t...
Development and characterization of a new plasmid delivery system based on chit...
Cadete, A.; Figueiredo, L.; Lopes, R.; Calado, Cecília; Almeida, A. J.; Gonçalves, L. M. D.Chitosan is one of the most promising polymers for drug delivery through the mucosal routes because of its polycationic, biocompatible, and biodegradable nature, and particularly due to its mucoadhesive and permeation-enhancing properties. Bile salts are known to interact with lipid membranes, increasing their permeability. The addition of bile salts to chitosan matrices may improve the delivery characteristics...
Staphylococcus epidermidis biofilms with higher proportions of dormant bacteria...
Cerca, Filipe; Andrade, Filipa; França, Ângela; Bonifácio, Elva; Ribeiro, Adília; Almeida, A. J.; Cerca, Nuno; Pier, Gerald B.; Azeredo, JoanaStaphylococcus epidermidis is an opportunistic pathogen due to its ability to establish biofilms on indwelling medical devices. The presence of high amounts of dormant bacteria is a hallmark of biofilms, making them more tolerant to antimicrobials and to the host immune response. We observed that S. epidermidis biofilms grown in excess glucose accumulated high amounts of viable but non-culturable (VBNC) bacteri...
Application of flow cytometry for the identification of Staphylococcus epidermi...
Azevedo, N. F.; Jardim, T.; Almeida, Carina; Cerqueira, L.; Almeida, A. J.; Rodrigues, Fernando José dos Santos; Keevil, C. W.; Vieira, M. J.Staphylococcus epidermidis is considered to be one of the most common causes of nosocomial bloodstream infections, particularly in immune-compromised individuals. Here, we report the development and application of a novel peptide nucleic acid probe for the specific detection of S. epidermidis by fluorescence in situ hybridization. The theoretical estimates of probe matching specificity and sensitivity were 89 a...
The C allele of rs5743836 polymorphism in the human TLR9 promoter links IL-6 an...
Carvalho, Agostinho; Osório, Nuno S.; Saraiva, Margarida; Cunha, Cristina; Almeida, A. J.; Coelho, Maria Teixeira; Ludovico, Paula; Pedrosa, JorgeIn humans, allelic variants in Toll-like receptors (TLRs) associate with several pathologies. However, the underlying cellular and molecular mechanisms of this association remain largely unknown. Analysis of the human TLR9 promoter revealed that the C allele of the rs5743836 polymorphism generates several regulatory sites, including an IL-6-responding element. Here, we show that, in mononuclear cells carrying t...
Staphylococcus epidermidis biofilms with higher proportions of dormant bacteria...
Cerca, Filipe; Andrade, Filipa; França, António; Bonifácio, Elva; Ribeiro, Adília; Almeida, A. J.; Cerca, Nuno; Pier, Gerald B.; Azeredo, JoanaStaphylococcus epidermidis an opportunistic pathogen due to its ability to establish biofilms on indwelling medical devices. The presence of high amounts of dormant bacteria is a hallmark of biofilms, making them more tolerant to antimicrobials and to the host immune response. We observed that S. epidermidis biofilms grown in excess glucose accumulated high amounts of viable but non-culturable (VBNC) bacteria, ...